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首页> 外文期刊>The Journal of Antibiotics: An International Journal >Two 18-membered epothilones from Sorangium cellulosum So0157-2
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Two 18-membered epothilones from Sorangium cellulosum So0157-2

机译：两个来自Sorangium cellulosum So0157-2的18元埃坡霉素

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摘要

Epothilones are 16-membered ring macrolides originally isolated from the bacterium Sorangiwn cellulosum Soce90, which were found to kill dividing cells by stabilizing microtubules. Previously, epothilone-producing strains of the genus Sorangiwn were isolated and screened in our laboratories. We found that one strain, designated S. cellulosum SoO 157-2, was able to produce epothilones. The production of epothilones A and В by S. cellulosum SoO 157-2 was optimized to industrial scales. Recently, five new epothilone glycoside derivatives were isolated from this strain. As a part of our ongoing search for new epothilones from S. cellulosum SoO 157-2, we focused on the minor and polar components of this strain produced in large-scale fermentations. The strain S. cellulosum So0157-2 was cultivated in 701 of M26 medium. Epohtilones A9 (3) (3.0 mg), A (4) (90 mg), В (250 mg) and С (20 mg) were isolated from the 701 fermentation extract by repeated column chromatography (Sephadex LH-20, RP-18 and silica gel, Sephadex, Uppsala, Sweden).
机译：上皮内酯是最初从细菌Sorangiwncellulosum Soce90分离的16元环大环内酯类化合物，被发现可通过稳定微管杀死分裂细胞。以前，在我们的实验室中分离并筛选了产生埃坡霉素的Sorangiwn属菌株。我们发现一种名为S. cellulosum SoO 157-2的菌株能够产生埃博霉素。 S. cellulosum SoO 157-2生产埃坡霉素A和В的规模已达到工业规模。最近，从该菌株中分离出五个新的埃坡霉素配糖衍生物。在我们持续的研究中，我们从纤维素酶SoO 157-2中寻找新的埃坡霉素，我们将重点放在大规模发酵中产生的该菌株的微量和极性成分上。在701 M26培养基中培养纤维素酶链球菌So0157-2。通过重复柱色谱法（Sephadex LH-20，RP-18）从701发酵提取物中分离出Epophtilones A9（3）（3.0 mg），A（4）（90 mg），В（250 mg）和С（20 mg）和硅胶（Sephadex，瑞典乌普萨拉）。

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