Bacteriostatic versus bactericidal activity of ciprofloxacin in Escherichia coli assessed by flow cytometry using a novel far-red dye.

Silva F; Lourenco O; Queiroz JA; Domingues FC

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Bacteriostatic versus bactericidal activity of ciprofloxacin in Escherichia coli assessed by flow cytometry using a novel far-red dye.

机译：环丙沙星在大肠杆菌中的抑菌活性与杀菌活性通过使用新型远红染料的流式细胞术评估。

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As common microbiological methods for the assessment of bacteriostatic or bactericidal activities are very time-consuming, in this work we describe that the use of a novel far-red fluorescent stain, Vybrant DyeCycle Ruby (DCR) for the flow cytometric analysis of fluoroquinolone (ciprofloxacin) bacteriostatic and bactericidal activities in Escherichia coli proved to be specific for bacterial DNA and, after ciprofloxacin exposure, DNA distribution analysis was achieved using a 7.5 muM DCR concentration to stain 5 x 10 ethanol-fixed bacterial cells. The analysis of the bacterial DNA histograms obtained from the ciprofloxacin concentrations tested, enabled the distinction between ciprofloxacin bacteriostatic and bactericidal activities.

机译：由于用于评估抑菌或杀菌活性的常用微生物方法非常耗时，因此在这项工作中，我们描述了使用新型远红色荧光染料Vybrant DyeCycle Ruby（DCR）对氟喹诺酮（环丙沙星）进行流式细胞术分析）大肠杆菌中的抑菌和杀菌活性被证明对细菌DNA具有特异性，在暴露于环丙沙星后，使用7.5μMDCR浓度对5 x 10乙醇固定的细菌细胞进行染色可实现DNA分布分析。从测试的环丙沙星浓度获得的细菌DNA直方图的分析实现了环丙沙星抑菌活性和杀菌活性之间的区别。

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《The Journal of Antibiotics: An International Journal》 |2011年第4期|共5页
作者
Silva F; Lourenco O; Queiroz JA; Domingues FC;
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1. Bacteriostatic versus bactericidal activity of ciprofloxacin in Escherichia coli assessed by flow cytometry using a novel far-red dye. [J] . Silva F, Lourenco O, Queiroz JA, The Journal of Antibiotics: An International Journal . 2011,第4期

机译：环丙沙星在大肠杆菌中的抑菌活性与杀菌活性通过使用新型远红染料的流式细胞术评估。
2. Bacteriostatic versus bactericidal activity of ciprofloxacin in Escherichia coli assessed by flow cytometry using a novel far-red dye. [J] . Silva F, Lourenco O, Queiroz JA, The Journal of Antibiotics: An International Journal . 2011,第4期

机译：用新型远红染料通过流式细胞术评估的大肠杆菌在大肠杆菌中的抑菌耐药性。
3. Bacteriostatic versus bactericidal activity of ciprofloxacin in Escherichia coli assessed by flow cytometry using a novel far-red dye. [J] . Silva F, Lourenco O, Queiroz JA, The Journal of Antibiotics: An International Journal . 2011,第4期

机译：用新型远红染料通过流式细胞术评估的大肠杆菌在大肠杆菌中的抑菌耐药性。
4. Effect of Chitosan and Alginate Concentration on Size and Bactericidal Activity against Escherichia coli of Chitosan/Alginate/Silver Nanoparticle Beads [C] . Pawika Mahasawat, Ketsarin Hlongkeaw, Sutthida Charoenrit Uttaradit Rajabhat University International Conference on Science and Technology . 2017

机译：壳聚糖及海藻酸盐浓度对壳聚糖/藻酸盐/银纳米粒子珠粒大肠杆菌大小和杀菌活性的影响
5. Bactericidal antibiotics induce nitric oxide production and signaling in Escherichia coli. [D] . Lawrence, Carolyn Anna. 2010

机译：杀菌抗生素在大肠杆菌中诱导一氧化氮的产生和信号传导。
6. Target for bacteriostatic and bactericidal activities of beta-lactam antibiotics against Escherichia coli resides in different penicillin-binding proteins. [O] . G Satta, G Cornaglia, A Mazzariol, 1995

机译：β-内酰胺类抗生素对大肠杆菌的抑菌和杀菌活性的目标位于不同的青霉素结合蛋白中。
7. Bacteriostatic versus bactericidal activity of ciprofloxacin in Escherichia coli assessed by flow cytometry using a novel far-red dye [O] . Filomena Silva, Olga Lourenço, João A Queiroz, 2011

机译：流式细胞仪评估使用新型远红染料的大肠杆菌中的菌种抗菌性活性
8. In vitro Activity of Ciprofloxacin Compared to Trimethoprim-Sulfamethoxazole Against Campylobacter Spp., Shigella Spp. and Enterotoxigenic Escherichia Coli Causing Traveller's Diarrhea in Egypt [R] . Mikhail, I. A., Bougeois, A. L., Hyams, K. C., 1987

机译：环丙沙星与甲氧苄氨嘧啶 - 磺胺甲恶唑对抗弯曲杆菌，志贺氏菌的体外活性产肠毒素大肠埃希氏菌导致埃及旅行者腹泻

Bacteriostatic versus bactericidal activity of ciprofloxacin in Escherichia coli assessed by flow cytometry using a novel far-red dye.

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