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首页> 外文期刊>The Journal of Antibiotics: An International Journal >ACT-6, a novel plasmid-encoded class C beta-lactamase in a Klebsiella pneumoniae isolate from China.
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ACT-6, a novel plasmid-encoded class C beta-lactamase in a Klebsiella pneumoniae isolate from China.

机译：ACT-6是一种来自中国肺炎克雷伯菌的新型质粒编码的C类β-内酰胺酶。

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摘要

The purpose of this study was to investigate the phenotypic and molecular characterization of a novel plasmid-mediated AmpC-type beta-lactamase in Klebsiella pneumoniae E701 isolated from Anhui province in China. In comparison with the ACT-1, sequence analysis revealed that there were 43 point mutations in the coding gene, and 10 of which led to amino-acid substitution. Resistance could be transferred by conjugation or transformation with plasmid DNA into E. coli JM109, which was due to the production of a beta-lactamase with an isoelectric point of 8.4 named ACT-6. Cloning, expression, purification and kinetics were carried out to study the characterization of the novel AmpC-type beta-lactamase. The results of MIC determinations and substrate profiles showed there was no significant difference in the activities of the novel enzyme and ACT-1. Moreover, the class 1 integron and the whole open reading frame of the novel AmpC-type beta-lactamase from K.pneumoniae E701 were detectable in the same size plasmid. This is the first report on the emergence of the novel ACT-6 type beta-lactamases in K. pneumoniae.
机译：本研究的目的是调查分离自中国安徽省的肺炎克雷伯菌E701中新型质粒介导的AmpC型β-内酰胺酶的表型和分子表征。与ACT-1相比，序列分析显示编码基因中存在43个点突变，其中10个导致氨基酸取代。可以通过与质粒DNA偶联或转化将抗性转移到大肠杆菌JM109中，这是由于产生了等电点为8.4的β-内酰胺酶，称为ACT-6。进行了克隆，表达，纯化和动力学研究，以研究新型AmpC型β-内酰胺酶的特性。 MIC测定和底物谱的结果表明，新型酶和ACT-1的活性没有显着差异。此外，在相同大小的质粒中可检测到来自肺炎克雷伯菌E701的新型AmpC型β-内酰胺酶的1类整合子和整个开放阅读框。这是关于肺炎克雷伯菌中新型ACT-6型β-内酰胺酶的首次报道。

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