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首页> 外文期刊>The Journal of Antibiotics: An International Journal >Comparison of drug sensitivity and genotypes of clinically isolated strains of levofloxacin-resistant Streptococcus pneumoniae obtained from Okinawa Island, the Japanese main island and Hong Kong.
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Comparison of drug sensitivity and genotypes of clinically isolated strains of levofloxacin-resistant Streptococcus pneumoniae obtained from Okinawa Island, the Japanese main island and Hong Kong.

机译：从冲绳岛，日本主岛和香港获得的耐左氧氟沙星肺炎链球菌临床分离株的药物敏感性和基因型比较。

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摘要

The prevalence of fluoroquinolone-resistant Streptococcus pneumoniae is increasing worldwide. In the present study, a comparison of drug sensitivity and genotypes of clinically isolated strains of levofloxacin (LVFX)-resistant S. pneumoniae obtained from Hong Kong, Okinawa Island and the Japanese main island (Honshu) was performed. MICs of quinolones (LVFX, tosufloxacin, ciprofloxacin, gatifloxacin and sitafloxacin (STFX)) and other antibiotics (penicillin G, cefcapene, cefditoren, clarithromycin and azithromycin) were determined by a microdilution broth method according to the Clinical and Laboratory Standards Institute Standards. The quinolone-resistance determining regions (QRDRs) of gyrA, gyrB, parC and parE of these strains were analyzed by PCR-based sequencing. All 40 strains tested had more than one amino-acid substitution in the QRDRs of gyrA, gyrB, parC or parE. Although there seemed to be some clonality in strains obtained from Hong Kong, there was no clonality in strains obtained from Okinawa and Japan. Strains obtained from Hong Kong, Okinawa Island and the Japanese main island were genetically different by pulsed-field gel electrophoresis analysis. The range of MIC values of STFX against isolates resistant to LVFX (MIC 4-32 mg l(-1)) was 0.12-0.5 mg l(-1), and MIC(80) values of STFX against LVFX-resistant isolates were 0.25 mg l(-1). This study suggests that LVFX-resistant S. pneumoniae is similar in all three locations and STFX is potent against LVFX-resistant S. pneumoniae with multiple mutations in QRDRs of gyrase A and topoisomerase IV.
机译：耐氟喹诺酮的肺炎链球菌在世界范围内呈上升趋势。在本研究中，对从香港，冲绳岛和日本主岛（本州）获得的耐左氧氟沙星（LVFX）肺炎链球菌临床分离株的药物敏感性和基因型进行了比较。喹诺酮类药物（LVFX，托氟沙星，环丙沙星，加替沙星和西他沙星（STFX））和其他抗生素（青霉素G，头孢卡芬，头孢托仑，克拉霉素和阿奇霉素）的MIC均根据临床和实验室标准通过微稀释液法测定。通过基于PCR的测序分析这些菌株的gyrA，gyrB，parC和parE的喹诺酮抗性确定区域（QRDR）。测试的所有40个菌株在gyrA，gyrB，parC或parE的QRDR中具有一个以上的氨基酸取代。尽管从香港获得的菌株似乎有些克隆性，但从冲绳和日本获得的菌株没有克隆性。通过脉冲场凝胶电泳分析，从香港，冲绳岛和日本主岛获得的菌株具有遗传差异。 STFX对耐LVFX的菌株的MIC值范围（MIC 4-32 mg l（-1））为0.12-0.5 mg l（-1），STFX对耐LVFX的菌株的MIC（80）值为0.25毫克升（-1）。这项研究表明，耐LVFX的肺炎链球菌在所有三个位置都相似，STFX对耐LVFX的肺炎链球菌具有强烈的回旋酶A和拓扑异构酶IV QRDR突变的作用。

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