...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The Journal of Antibiotics: An International Journal >Recognition of acyl carrier proteins by ketoreductases in assembly line polyketide synthases
【24h】

Recognition of acyl carrier proteins by ketoreductases in assembly line polyketide synthases

机译：装配线聚酮化合物合酶中酮还原酶对酰基载体蛋白的识别

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Ketoreductases (KRs) are the most widespread tailoring domains found in individual modules of assembly line polyketide synthases (PKSs), and are responsible for controlling the configurations of both the alpha-methyl and beta-hydroxyl stereogenic centers in the growing polyketide chain. Because they recognize substrates that are covalently bound to acyl carrier proteins (ACPs) within the same PKS module, we sought to quantify the extent to which protein-protein recognition contributes to the turnover of these oxidoreductive enzymes using stand-alone domains from the 6-deoxyerythronolide B synthase (DEBS). Reduced 2-methyl-3-hydroxyacyl-ACP substrates derived from two enantiomeric acyl chains and four distinct ACP domains were synthesized and presented to four distinct KR domains. Two KRs, from DEBS modules 2 and 5, displayed little preference for oxidation of substrates tethered to their cognate ACP domains over those attached to the other ACP domains tested. In contrast, the KR from DEBS module 1 showed an similar to 10-50-fold preference for substrate attached to its native ACP domain, whereas the KR from DEBS module 6 actually displayed an similar to 10-fold preference for the ACP from DEBS module 5. Our findings suggest that recognition of the ACP by a KR domain is unlikely to affect the rate of native assembly line polyketide biosynthesis. In some cases, however, unfavorable KR-ACP interactions may suppress the rate of substrate processing when KR domains are swapped to construct hybrid PKS modules.
机译：酮还原酶（KRs）是在组装线聚酮化合物合酶（PKS）的各个模块中发现的最广泛的定制域，负责控制生长中的聚酮化合物链中α-甲基和β-羟基立体异构中心的构型。由于它们可以识别与相同PKS模块内的酰基载体蛋白（ACP）共价结合的底物，因此我们尝试使用6-结构域中的独立域来量化蛋白质-蛋白质识别对这些氧化还原酶营业额的贡献程度。脱氧赤藓醇B合酶（DEBS）。合成了来自两个对映体酰基链和四个不同的ACP域的还原的2-甲基-3-羟酰基-ACP底物，并提供给四个不同的KR域。来自DEBS模块2和5的两个KR与连接到其他测试ACP域的底物相比，对于拴系在其同源ACP域上的底物的氧化显示出较小的偏爱。相比之下，来自DEBS模块1的KR对附着到其天然ACP域的底物表现出相似的10-50倍偏好，而来自DEBS模块6的KR实际上对来自DEBS模块的ACP表现出相似于10倍的偏好。 5.我们的发现表明，KR域识别ACP不太可能影响天然组装线聚酮化合物的生物合成速率。但是，在某些情况下，当将KR域互换来构建混合PKS模块时，不利的KR-ACP相互作用可能会抑制底物处理的速率。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号