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首页> 外文期刊>The Journal of Antimicrobial Chemotherapy >Macrolide resistance and erythromycin resistance determinants among Belgian Streptococcus pyogenes and Streptococcus pneumoniae isolates.
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Macrolide resistance and erythromycin resistance determinants among Belgian Streptococcus pyogenes and Streptococcus pneumoniae isolates.

机译：比利时化脓性链球菌和肺炎链球菌分离株中的大环内酯类耐药性和红霉素耐药性决定因素。

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摘要

Resistance of streptococci to macrolide antibiotics is caused by target-site modification or drug efflux. The phenotypic expression of target-site modification can be inducible or constitutive. The prevalence of the three phenotypes among Belgian erythromycin-resistant Group A streptococci (GAS) and Streptococcus pneumoniae isolates was surveyed, their MICs for seven antibiotics were determined and the clonality of the isolates was explored. Of the 2014 GAS isolates tested 131(6.5%) were erythromycin resistant (MIC > 1 mg/L): 110 (84.0%) showed the M-resistance phenotype whereas the remaining 21 strains (16.0%) were constitutively resistant. No inducibly resistant strains were detected. Of 100 S. pneumoniae isolates, 33 were erythromycin resistant (MIC > 1 mg/L). In contrast to the GAS isolates, only 9.1% of the 33 erythromycin-resistant S. pneumoniae isolates showed the M-resistance phenotype. The presence of mefA/E and ermB genes in the M-resistant and constitutively and inducibly resistant strains, respectively, was confirmed by PCR analysis. Genomic analysis based on pulsed-field gel electrophoresis (PFGE) using the restriction enzyme SfiI, revealed 54 different PFGE patterns among the 131 erythromycin-resistant GAS isolates, of which an M6 clone represented 16.0% of the strains; all other clones, exhibiting different M-types, represented <7% of the strains. The S. pneumoniae isolates also appeared to be polyclonally based, as determined by arbitrarily primed PCR. The macrolides miocamycin and rovamycin, the lincosamide clindamycin and the ketolide HMR 3647 showed excellent activity against the M-resistant GAS and S. pneumoniae strains.
机译：链球菌对大环内酯类抗生素的耐药性是由靶位修饰或药物外排引起的。靶位点修饰的表型表达可以是诱导型的或组成型的。调查了比利时抗红霉素的A组链球菌（GAS）和肺炎链球菌分离株这3个表型的患病率，确定了7种抗生素的MIC，并对分离株的克隆性进行了研究。在2014年测试的GAS菌株中，有131（6.5％）种对红霉素具有抗性（MIC> 1 mg / L）：110（84.0％）具有M抗性表型，而其余21株（16.0％）具有组成型抗性。未检测到诱导抗性菌株。在100株肺炎链球菌中，有33株对红霉素耐药（MIC> 1 mg / L）。与GAS分离株相反，在33株对红霉素耐药的肺炎链球菌分离株中，只有9.1％表现出M耐药表型。通过PCR分析证实了M抗性和组成型和诱导型抗性菌株中分别存在mefA / E和ermB基因。基于使用限制酶SfiI的脉冲场凝胶电泳（PFGE）进行的基因组分析显示，在131株抗红霉素的GAS菌株中，有54种不同的PFGE模式，其中M6克隆占菌株的16.0％;表现出不同M型的所有其他克隆占菌株的<7％。肺炎链球菌分离株也似乎是基于多克隆的，如通过任意引物PCR确定的。大环内酯类米卡霉素和罗瓦霉素，林可酰胺克林霉素和酮内酯HMR 3647对M耐药的GAS和肺炎链球菌菌株表现出出色的活性。

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