...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The Journal of Antimicrobial Chemotherapy >Tetracycline resistance genes in isolates of Pasteurella multocida, Mannheimia haemolytica, Mannheimia glucosida and Mannheimia varigena from bovine and swine respiratory disease: intergeneric spread of the tet(H) plasmid pMHT1.
【24h】

Tetracycline resistance genes in isolates of Pasteurella multocida, Mannheimia haemolytica, Mannheimia glucosida and Mannheimia varigena from bovine and swine respiratory disease: intergeneric spread of the tet(H) plasmid pMHT1.

机译：牛和猪呼吸系统疾病中多杀巴斯德氏菌，溶血曼海姆氏杆菌，葡萄糖曼尼曼氏菌和变异曼尼曼氏菌分离株的四环素抗性基因：tet（H）质粒pMHT1的种间传播。

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Tetracycline-resistant isolates of Pasteurella multocida and Mannheimia spp. from respiratory diseases in cattle and swine were investigated for the classes of tet gene and their chromosomal or plasmid location. The 34 isolates comprised eight P. multocida, 23 Mannheimia haemolytica, two Mannheimia varigena and a single Mannheimia glucosida isolate. Identification of the tet genes was achieved by PCR analysis and hybridization with specific probes. Transformation and hybridization experiments served to confirm the plasmid location of tet genes. Selected tet genes and their adjacent regions were sequenced. The tet genes tet(B), tet(G) and tet(H) were detected. The gene tet(H) was present in 26 isolates. The 4.4 kb tet(H)-carrying plasmid pMHT1 was detected in six isolates representing all four species. In the remaining 28 isolates, copies of tet(B), tet(G) and tet(H) were identified as chromosomal. No correlation between the tet gene type and the MIC of tetracycline, or between the number of tet gene copies and the MIC of tetracycline was observed. Tetracycline resistance in P. multocida and Mannheimia spp. is mediated by at least three different tet genes. A new type of tet(H)- carrying plasmid, pMHT1, was identified. The detection of pMHT1 in M. glucosida and M. varigena is the first report of resistance plasmids in isolates of these two species. For the first time, tet(G) genes were detected in members of the family Pasteurellaceae.
机译：多杀性巴斯德氏菌和曼海姆氏菌的四环素抗性分离株。从牛和猪的呼吸道疾病中研究了tet基因的种类及其染色体或质粒位置。这34个分离物包括8个多杀性巴氏杆菌，23个溶血曼海姆氏菌，2个曼氏海默氏菌和1个曼氏葡糖苷分离物。通过PCR分析和与特异探针的杂交来实现tet基因的鉴定。转化和杂交实验用于确认tet基因的质粒位置。对选定的tet基因及其邻近区域进行测序。检测到tet基因tet（B），tet（G）和tet（H）。基因tet（H）存在于26个分离株中。在代表所有四个物种的六个分离物中检测到携带4.4 kb tet（H）的质粒pMHT1。在其余的28个分离株中，tet（B），tet（G）和tet（H）的拷贝被鉴定为染色体。在tet基因类型与四环素的MIC之间，或在tet基因拷贝数与四环素的MIC之间未发现相关性。 P. multocida和Mannheimia spp中的四环素抗性。由至少三个不同的tet基因介导。鉴定了一种新型的携带tet（H）的质粒pMHT1。在这两个物种的分离株中首次发现抗性质粒pGHT1是在葡糖葡萄球菌和变异型葡萄球菌中。首次在巴氏杆菌科成员中检测到tet（G）基因。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号