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首页> 外文期刊>The Journal of Antimicrobial Chemotherapy >Phenotypic and genetic characterization of the 2008 WHO Neisseria gonorrhoeae reference strain panel intended for global quality assurance and quality control of gonococcal antimicrobial resistance surveillance for public health purposes.
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Phenotypic and genetic characterization of the 2008 WHO Neisseria gonorrhoeae reference strain panel intended for global quality assurance and quality control of gonococcal antimicrobial resistance surveillance for public health purposes.

机译：2008年世卫组织淋病奈瑟氏球菌参考菌株小组的表型和遗传特征，旨在为公共卫生目的对全球淋球菌抗菌素耐药性进行质量保证和质量控制。

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OBJECTIVES: Emergence and spread of antimicrobial resistance (AMR) in Neisseria gonorrhoeae remain a major global problem and expanded, but valid, AMR surveillance is crucial for public health purposes. The World Health Organization (WHO) Collaborating Centre in Sydney, Australia, continually evaluates N. gonorrhoeae strains used in quality control and assurance aspects of the national, WHO regional and international programmes for AMR surveillance it conducts. Here we phenotypically and genetically characterized the 2008 WHO N. gonorrhoeae reference panel, widely used under existing WHO AMR surveillance protocols. MATERIALS AND METHODS: The eight N. gonorrhoeae WHO reference strains were phenotypically characterized by antibiogram, auxotype, serovar and prolyliminopeptidase screening; and genetically with regard to resistance plasmid types, polymorphisms in divergent genetic resistance-mediating loci (n = 9), porB sequencing and N. gonorrhoeae multi-antigen sequence typing. RESULTS: The 2008 WHO reference strains represented all the important susceptible and resistant phenotypes, including corresponding resistance genotypes, and the range of resistances currently seen for relevant antimicrobials. Several pertinent additional phenotypic and genotypic markers, for example, epidemiological markers, were also determined. CONCLUSIONS: The 2008 WHO N. gonorrhoeae reference strain panel was extensively characterized, which is crucial for the expansion of gonococcal AMR surveillance nationally and internationally. The panel is available through WHO sources for quality assurance and quality control aspects of current phenotypic testing protocols, to allow valid comparison of AMR data derived by divergent methods, and also for the control of present and future molecular assays for AMR detection. Additional WHO reference strains can be included as required by the emergence of additional resistant phenotypes and/or genotypes.
机译：目的：淋病奈瑟氏球菌的耐药性（AMR）的出现和扩散仍然是一个主要的全球性问题，并且扩大了范围，但有效的AMR监测对于公共卫生目的至关重要。位于澳大利亚悉尼的世界卫生组织（世卫组织）合作中心不断评估用于其国家，世卫组织区域和国际AMR监测计划的质量控制和保证方面的淋病奈瑟氏球菌。在这里，我们对2008年WHO淋病奈瑟氏球菌参考小组进行了表型和基因表征，该小组在现有WHO AMR监测方案下得到了广泛使用。材料与方法：对八株淋病奈瑟氏球菌WHO参考菌株进行了表型分析，抗菌谱，血型，血清型和脯氨肽酶的筛选。在遗传上，与抗性质粒类型，介导不同遗传抗性的基因座（n = 9）的多态性，porB测序和淋病奈瑟氏球菌多抗原序列分型有关。结果：2008 WHO参考菌株代表了所有重要的易感和耐药表型，包括相应的耐药基因型，以及目前有关抗菌素的耐药范围。还确定了其他一些相关的表型和基因型标记，例如流行病学标记。结论：2008年世界卫生组织淋病奈瑟氏球菌参考菌株小组被广泛鉴定，这对于在国内和国际上扩大淋球菌AMR监测至关重要。该小组可通过WHO来源获得，用于当前表型测试规程的质量保证和质量控制方面，以便有效比较通过不同方法得出的AMR数据，还可以控制当前和将来的AMR检测分子测定。其他抗性表型和/或基因型的出现可根据需要包括其他WHO参考菌株。

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