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首页> 外文期刊>The journal of maternal-fetal & neonatal medicine >Terbutaline inhibits corticotropin-releasing hormone (CRH) expression in human trophoblast cells.
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Terbutaline inhibits corticotropin-releasing hormone (CRH) expression in human trophoblast cells.

机译：特布他林抑制人滋养细胞中促肾上腺皮质激素释放激素（CRH）的表达。

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Objective. To evaluate the effect of beta-adrenergic agonists on the regulation of the expression of the placental corticotropin-releasing hormone (CRH) gene.Study design. Term placentae were collected at the time of elective cesarean section, and trophoblast cells were harvested, isolated, and cultured. The isolated trophoblasts were plated, cultured and subsequently treated with cortisol, terbutaline, RU486, or vehicle control. CRH expression, mRNA abundance of CRH, and the housekeeping gene beta-actin were evaluated by Northern blot analysis.Results. Exposure of the trophoblasts to terbutaline (10-8 M) inhibited the expression of the CRH gene as depicted by Northern blot analysis. Co-addition of terbutaline (10-8 M) and RU486 (10-6 M) did not block the stimulatory effects of RU486 on placental trophoblast cells.Conclusion. The beta-adrenergic agonist terbutaline inhibits the expression of CRH in human trophoblasts. This finding may provide insight into the mechanism of action of terbutaline as a tocolytic agent.
机译：目的。评估β-肾上腺素能激动剂对胎盘促肾上腺皮质激素释放激素（CRH）基因表达的调节作用。研究设计。在选择性剖宫产时收集足月胎盘，并收集，分离和培养滋养层细胞。将分离的滋养细胞铺板，培养并随后用皮质醇，特布他林，RU486或媒介物对照处理。通过Northern印迹分析评价CRH的表达，CRH的mRNA丰度和管家基因β-肌动蛋白。如Northern印迹分析所示，将滋养细胞暴露于特布他林（10-8 M）可抑制CRH基因的表达。特布他林（10-8 M）和RU486（10-6 M）的共同添加并未阻止RU486对胎盘滋养层细胞的刺激作用。 β-肾上腺素能激动剂特布他林抑制人滋养细胞中CRH的表达。该发现可以提供对特布他林作为宫缩抑制剂作用机理的见解。

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