...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The Journal of General and Applied Microbiology >Phylogenetic analysis of gamma-proteobacteria inferred from nucleotide sequence comparisons of the house-keeping genes adk,aroE and gdh: Comparisons with phylogeny inferred from 16S rRNA gene sequences
【24h】

Phylogenetic analysis of gamma-proteobacteria inferred from nucleotide sequence comparisons of the house-keeping genes adk,aroE and gdh: Comparisons with phylogeny inferred from 16S rRNA gene sequences

机译：从持家基因adk，aroE和gdh的核苷酸序列比较推断出的γ-变形细菌的系统发育分析：与从16S rRNA基因序列推断出的系统发育的比较

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Nucleotide sequence comparisons of three house-keeping genes,adenylate kinase (adk),shiki-mate dehydrogenase (aroE),and glucose-6-phosphate dehydrogenase (gdh),were used to infer the phylogeny of 33 gamma-proteobacteria.Phylogenetic trees inferred from each gene,and from the concatenated sequences of all three genes,are,in general,similar to a 16S rRNA gene-inferred tree.Similar grouping of bacteria are revealed at the family,genus,species and strain levels in all five trees.The house-keeping genes,however,show a higher rate of nucleotide sequence substitutions.Consequently,they can possibly probe deeper branches of a phylogenetic tree than the 16S rRNA gene.However,because their nucleotide sequences are not as highly conserved among gamma-proteobacteria,family- or genus-specific primers would need to be designed for the amplification of any of these three house-keeping genes.Since these genes are used in mul-tilocus sequence typing,it is expected that the number of sequences publicly available for many taxa will increase over time proving them very useful either at complementing 16S rRNA-inferred phylogenies or for specific,targeted,phylogenetic analysis.
机译：使用三个持家基因，腺苷酸激酶（adk），志趣伴侣脱氢酶（aroE）和葡萄糖-6-磷酸脱氢酶（gdh）的核苷酸序列比较来推断33种γ-变形杆菌的系统发育。通常，来自每个基因以及来自所有三个基因的连接序列与16S rRNA基因推导的树相似。在这五棵树的科，属，种和菌株水平上都揭示了相似的细菌分组。然而，管家基因显示出更高的核苷酸序列取代率。因此，它们可能比16S rRNA基因探测系统树的更深分支。但是，由于它们的核苷酸序列在γ-变形杆菌中并不那么保守。 ，家族或属特异的引物需要设计用于扩增这三个持家基因中的任何一个。由于这些基因用于多基因序列分型，因此预计公开的序列数随着时间的推移，许多分类单元的可用量将增加，证明它们在补充16S rRNA推断的系统发育或进行特定的，有针对性的系统发育分析方面非常有用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号