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首页> 外文期刊>The journal of maternal-fetal & neonatal medicine >Wnt5a inhibited human trophoblast cell line HTR8/SVneo invasion: implications for early placentation and preeclampsia
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Wnt5a inhibited human trophoblast cell line HTR8/SVneo invasion: implications for early placentation and preeclampsia

机译：Wnt5a抑制人类滋养细胞HTR8 / SVneo侵袭：对早期胎盘和先兆子痫的影响

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摘要

Objective: Wnt5a and Wnt signaling play potential roles in human placental and fetal development. The objective of this study is to explore the role of Wnt5a in the invasion of the human trophoblast cell line HTR8/SVneo and the probable mechanism of early placentation and preeclampsia in which Wnt5a is involved.Methods: Human first trimester villous tissues from normal pregnancies and third trimester placentas from pregnancies with or without preeclampsia (PE) were used in the detection of the expression and subcellular location of Wnt5a. The human trophoblast cell line HTR8/SVneo was treated with 0-400ng/ml recombinant Wnt5a to investigate the role of Wnt5a in human trophoblast invasion.Results: Human first trimester villous is accompanied by the decreased expression of Wnt5a compared with term placenta. Upregulated Wnt5a was detected in PE placenta compared with the normal control. Wnt5a inhibited the migration and invasion of HTR8/SVneo cells with decreased integrin 1, 5 and N-cadherin. Moreover, Wnt5a downregulated -catenin in HTR8/SVneo cells.Conclusions: These findings strongly suggest that Wnt5a inhibits the invasion of HTR8/SVneo cells. Decreased Wnt5a facilitates early placentation, whereas increased Wnt5a contributes to the pathogenesis of PE with insufficient trophoblast invasion. Aberrant Wnt5a may function by impairing Wnt non-canonical/-catenin signaling pathway in trophoblasts.
机译：目的：Wnt5a和Wnt信号在人类胎盘和胎儿发育中发挥潜在作用。这项研究的目的是探讨Wnt5a在人类滋养层细胞HTR8 / SVneo侵袭中的作用以及Wnt5a参与的早期胎盘和子痫前期的可能机制。方法：正常妊娠和早孕的人类早孕绒毛组织来自妊娠有或没有先兆子痫（PE）的妊娠晚期胎盘被用于检测Wnt5a的表达和亚细胞定位。用0-400ng / ml重组Wnt5a处理人滋养层细胞HTR8 / SVneo，研究Wnt5a在滋养层细胞侵袭中的作用。结果：人早孕绒毛与Wnt5a表达相比足月胎盘减少。与正常对照相比，在PE胎盘中检测到Wnt5a上调。 Wnt5a抑制了整联蛋白1、5和N-钙黏着蛋白的表达，从而抑制了HTR8 / SVneo细胞的迁移和侵袭。此外，Wnt5a下调了HTR8 / SVneo细胞中的-catenin。结论：这些发现强烈表明Wnt5a抑制HTR8 / SVneo细胞的侵袭。 Wnt5a的减少有助于早期胎盘形成，而Wnt5a的增加则导致滋养层浸润不足而引起PE的发病。异常的Wnt5a可能通过破坏滋养细胞中的Wnt非规范/连环蛋白信号通路而发挥作用。

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