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首页> 外文期刊>The Journal of Comparative Neurology >Vesicular glutamate transporter-3 in the rodent brain: Vesicular colocalization with vesicular γ-aminobutyric acid transporter
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Vesicular glutamate transporter-3 in the rodent brain: Vesicular colocalization with vesicular γ-aminobutyric acid transporter

机译：啮齿类动物脑中的囊泡谷氨酸转运蛋白-3：与囊泡γ-氨基丁酸转运蛋白的囊泡共定位

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摘要

Vesicular glutamate transporters (VGLUT1-3) carry glutamate into synaptic vesicles. VGLUT3 has been reported to be localized in nonglutamatergic neuronal populations in the brain. However, detailed subcellular localization of VGLUT3 has not been shown. In particular, the identity of synaptic vesicles expressing VGLUT3 remains to be revealed. Here we present novel electron microscopic postembedding immunogold data from mouse and rat brains showing that small, clear, and round synaptic vesicles in γ-aminobutyric acid (GABA)-ergic nerve terminals contain labeling for both VGLUT3 and the vesicular GABA transporter (VGAT). Immunoisolation of synaptic vesicles confirmed the immunogold data and showed vesicular colocalization of VGLUT3 and VGAT. Moreover, we show that gold particles signaling VGLUT3 are present in synaptic vesicles in acetylcholinergic nerve terminals in the striatum. Quantitative immunogold analyses reveal that the density of VGLUT3 gold particles is similar in GABAergic terminals in the hippocampus and the neocortex to that in cholinergic terminals in the striatum. In contrast to in the hippocampus and the neocortex, VGLUT3 was absent from VGAT-positive terminals in the striatum. The labeling pattern produced by the VGLUT3 antibodies was found to be specific; there was no labeling in VGLUT3 knockout tissue, and the observed labeling throughout the rat brain corresponds to the known light-microscopic distribution of VGLUT3. From the present results, we infer that glutamate is released with GABA from inhibitory terminals and acetylcholine from cholinergic terminals. J. Comp. Neurol. 521: 3042-3056, 2013.
机译：囊状谷氨酸转运蛋白（VGLUT1-3）将谷氨酸转运到突触小泡中。据报道VGLUT3定位在大脑的非谷氨酸能神经元群体中。但是，尚未显示VGLUT3的详细亚细胞定位。特别是，表达VGLUT3的突触小泡的身份仍有待揭示。在这里，我们介绍了来自小鼠和大鼠大脑的新型电子显微镜植入后免疫金的数据，表明在γ-氨基丁酸（GABA）的神经末梢中小的，清晰且圆形的突触小泡包含VGLUT3和囊泡GABA转运蛋白（VGAT）的标记。突触小泡的免疫隔离证实了免疫金数据，并显示了VGLUT3和VGAT的小泡共定位。此外，我们表明信号VGLUT3的金颗粒存在于纹状体中乙酰胆碱能神经末梢的突触小泡中。免疫金的定量分析表明，VGLUT3金颗粒的密度在海马和新皮层的GABA能端与纹状体胆碱能端相似。与海马和新皮质相反，纹状体中VGAT阳性末端不存在VGLUT3。发现由VGLUT3抗体产生的标记模式是特异性的。在VGLUT3基因敲除组织中没有标记，并且在整个大鼠脑中观察到的标记对应于VGLUT3的已知光学显微镜分布。从目前的结果，我们推断谷氨酸与抑制末端的GABA释放，而胆碱能末端的乙酰胆碱释放。 J.比较神经元。 521：3042-3056，2013。

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