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首页> 外文期刊>The Journal of Horticultural Science & Biotechnology >In vitro performance at high culture pH and in vivo responses to Fe-deficiency of leaf-derived quince BA 29 (Cydonia oblonga) somaclones regenerated at variable medium pH
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In vitro performance at high culture pH and in vivo responses to Fe-deficiency of leaf-derived quince BA 29 (Cydonia oblonga) somaclones regenerated at variable medium pH

机译：高培养液pH值下的体外表现以及对叶衍生的木瓜BA 29（长扁藻）Somaclones在不同培养基pH下的体内对铁缺乏的响应

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摘要

The aims of the work were to obtain quince (Cydonia oblonga) BA 29 somaclones tolerant to high culture pH and lime soils, to test the effectiveness in vitro selection methods based on the selection pressure exerted by pH during regeneration, and to understand the mechanisms for the improved performance under Fe-deficient conditions of eventually tolerant clones. Leaf-derived somaclones obtained at variable medium pH (5.7, 6.0, 6.5, 7.0 and 8.0) were compared with each other and with the control (i.e. shoots derived from BA 29 mother plants by standard micropropagation) for their in vitro growth, proliferation, rooting performance and pH-reducing ability at pH 7.0, 7.5 and 8.0 and for their Fe-chelate reductase (FCR) activity and soluble sugar and organic acid accumulation in the roots and leaf chlorophyll content when grown in soiless culture under Fe-efficient and -deficient conditions. Preliminary results on SPAD analysis on leaves of the same plants transferred to lime soil are also reported. The effect of pH selection pressure was not very clear; however, while all the clones hardly grew and proliferated in vitro at pH 8, the somaclones regenerated at pH 7 generally tended to have better growth and proliferation than control and other somaclones at pH 7.5, especially 7-A. In contrast, most of those regenerated at low pH, but 5.7-A, tended to have lower proliferation rates and shorter axillary shoots. Little differences were found in the rooting performances of the clones, although most somaclones seemed more able to reduce the pH than the control. After 10 d in hydroponic culture under Fe-deficient conditions, almost all the somaclones tended to have some FCR induction in the roots; however, it was detected only in 5.7-A and 7-A at day 30. Leaf chlorophyll determinations in soiless culture and lime soil indicated quite good tolerance to Fe-chlorosis for 6-A, 7-A and especially 5.7-A. When the in vitro and in vivo results are considered together, the somaclones 5.7-A, 7-A and 6-A seem the most interesting, their success in growing in Fe-deficient conditions being probably due partially to sorbitol accumulation in the roots, in particular for 6-A and 5.7-A, and also to inositol and citric acid, respectively for 5.7-A and 7-A. However, further plant evaluation on lime soil is needed to verify these preliminary results and to understand whether some of these somaclones can replace the widespread pear rootstock quince BA 29, as more tolerant to lime-induced Fe-chlorosis.
机译：该工作的目的是获得耐高培养pH和石灰土壤的木瓜（Cydonia oblonga）BA 29体细胞克隆，基于再生过程中pH施加的选择压力来测试体外选择方法的有效性，并了解其机理。在铁缺乏条件下最终耐受克隆的性能提高。将在可变pH值（5.7、6.0、6.5、7.0和8.0）下获得的源自叶的somaclone彼此进行比较，并与对照（即通过标准微繁殖从BA 29母本植物衍生的芽）进行体外生长，增殖，在高铁和高效率的土壤条件下培养时，在pH 7.0、7.5和8.0下的生根性能和pH降低能力，以及它们在土壤中的Fe-螯合还原酶（FCR）活性和可溶性糖和有机酸在根中的积累以及叶绿素含量条件不足。还报道了对转移到石灰土壤的同一植物的叶片进行SPAD分析的初步结果。 pH选择压力的影响不是很明显。然而，尽管所有克隆几乎都无法在pH 8的条件下在体外生长和增殖，但在pH 7再生的体细胞克隆通常比在pH 7.5，尤其是7-A的对照和其他体细胞克隆具有更好的生长和增殖。相比之下，大多数在低pH值但5.7-A下再生的那些趋向于具有较低的增殖速率和较短的腋生芽。在克隆的生根性能中几乎没有发现差异，尽管大多数松果克隆似乎比对照更能降低pH。在缺铁条件下进行水耕培养10 d后，几乎所有体细胞无性激素都趋向于在根部产生一定的FCR诱导。但是，在第30天仅在5.7-A和7-A中检测到。在土壤无土栽培和石灰土壤中测定叶绿素表明，对6-A，7-A，尤其是5.7-A的铁绿叶病具有很好的耐受性。综合考虑体外和体内结果后，索莫克隆5.7-A，7-A和6-A似乎是最有趣的，它们在缺铁条件下生长的成功可能部分归因于山梨醇在根中的积累，特别是对于6-A和5.7-A，以及对于肌醇和柠檬酸，分别对于5.7-A和7-A。但是，还需要在石灰土壤上进行进一步的植物评估，以验证这些初步结果，并了解其中的某些无毒无草醇是否可以代替普遍存在的梨砧木木瓜BA 29，因为它们对石灰诱导的铁-绿化病的耐受性更高。

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