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首页> 外文期刊>The Journal of Horticultural Science & Biotechnology >Effect of temperature and light on rooting in vitro and subsequent flowering of pot alstroemria
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Effect of temperature and light on rooting in vitro and subsequent flowering of pot alstroemria

机译：温度和光照对六出木盆生根及随后开花的影响

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摘要

A clone of dwarf alstroemeria cv. Rosalina propagated in vitro was used in experiments to develop a method for pot alstroemeria production. The aim of this study was to optimize the in vitro rooting conditions of the rhizomes so as to obtain early flowering after transplanting to a greenhouse. The effects of cooling in darkness or in light, the addition of paclobutrazol to the rooting medium and the effect of stage of rhizome development on subsequent flowering were studied. The in vitro rhizome cultures maintained on the rooting medium were cooled at 10 deg C for 2-5 weeks and at 5 deg C for five weeks in darkness or under a 16 h photoperiod. To allow root formation and growth, the rhizomes were kept for 10 d at 20 deg C and 16 h photoperiod before cooling at 5 deg C and 10 deg C. Pretreatment with 20 deg C for 10 d and 16 h photoperiod applied during cooling resulted in superior rooting in vitro and better plant survival in the greenhouse. The faster flowering and most plants flowering were obtained after five weeks of cooling at 10 deg C under a 16 h photoperiod using pre-rooted rhizomes. Paclobutrazol markedly decreased flower stem length.
机译：矮六出cv的一个克隆。实验中使用了体外繁殖的蔷薇花，以开发一种生产六出花的方法。这项研究的目的是优化根茎的体外生根条件，以便在移栽到温室后获得早花。研究了在黑暗或光线下冷却，在根系培养基中添加多效唑的影响以及根茎发育阶段对随后开花的影响。保持在生根培养基上的体外根茎培养物在黑暗中或在16 h光周期下，在10摄氏度下冷却2-5周，在5摄氏度下冷却5周。为了使根形成和生长，根茎在20℃和16 h的光周期下保持10 d，然后在5℃和10℃冷却。在冷却过程中用20℃预处理10 d和16 h的光周期导致更好的生根和温室中更好的植物生存。使用预先生根的根茎，在10摄氏度，16小时的光照条件下冷却5周后，可以获得较快的开花和大多数植物的开花。多效唑明显减少了花茎的长度。

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