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首页> 外文期刊>The Journal of Horticultural Science & Biotechnology >Ammonium and nitrate assimilationin Narcissus roots
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Ammonium and nitrate assimilationin Narcissus roots

机译：水仙根中铵和硝酸盐的同化作用

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Narcissus cv. Garden Giant was cultivated in solution supplied with labelled - ~(15)NH_4~+ or ~(15) NO_3~-. The experiment was conducted with five treatments: T1: 1.0 mM~(15) NH_4~+ -N, T2: 1.0 mM~(15)NO_3~- -N, T3:0.5 mM ~NH_4~+ -N plus 0.5 mM NO_3~- -N, T4:0.5 mMNH_4~+ -N plus 0.05 mM~(15)NO_3~- -N and T5: N free solution. Samples were harvested at 2, 4 and 7 d after feeding (DAF). Ammonium-N (T1) was more rapidly absorbed by roots 2 d after feeding (DAF) than nitrate-N (T2). However, at 4-7 DAF, the amounts of N absorbed from ammonium-N and nitrate-N were almost equal, suggesting that narcissus roots can absorb and assimilate both forms of N. When combined forms of ammonium and nitrate were supplied, roots absorbed ammonium in preference to nitrate. The presence of ammonium depressed nitrate absorption and assimilation. Asparagine was the major free amino acid in roots, with N-free solution (T5). When plants were supplied with ammonium, the concentration of glutamine dramatically increased. With the single supply of nitrate, asparagine and glutamine accumulated to the same levels. ~(15)NH_4~+ was very actively assimilated into glutamine, and the presence of NH_4~+ depressed the assimilation of NO_3~- in the roots.
机译：水仙简历Garden Giant在标有-〜（15）NH_4〜+或〜（15）NO_3〜-的溶液中培养。实验进行了五种处理：T1：1.0 mM〜（15）NH_4〜+ -N，T2：1.0 mM〜（15）NO_3〜--N，T3：0.5 mM〜NH_4〜+ -N加0.5 mM NO_3 〜--N，T 4：0.5mMNH 4-+-N加上0.05mM〜（15）NO_3---N和T 5：无N的溶液。进食后第2、4和7天（DAF）收集样品。饲喂（DAF）后2 d，根系中的铵态氮（T1）比硝态氮（T2）吸收更快。但是，在4-7 DAF时，铵态氮和硝酸盐-N吸收的氮量几乎相等，这表明水仙根能够吸收和吸收两种形式的N。当铵态氮和硝酸盐混合供给时，根系吸收铵优先于硝酸盐。铵的存在降低了硝酸盐的吸收和吸收。天冬酰胺是根中的主要游离氨基酸，无氮溶液（T5）。向植物提供铵后，谷氨酰胺的浓度急剧增加。仅提供硝酸盐，天冬酰胺和谷氨酰胺积累到相同水平。 〜（15）NH_4〜+被非常活跃地吸收到谷氨酰胺中，而NH_4〜+的存在抑制了根中NO_3〜-的吸收。

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