• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The Journal of Horticultural Science & Biotechnology >Molecular tagging and mapping of a major linked marker for taproot thickness in a Brassica crop.
【24h】

Molecular tagging and mapping of a major linked marker for taproot thickness in a Brassica crop.

机译：甘蓝型作物中主根厚度的主要连锁标记的分子标记和作图。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A DNA marker linked to the locus controlling storage-root thickness was identified using bulked segregant analysis (BSA) in an F2 population derived from a cross between turnip (Brassica rapa L. ssp. rapifera Matzg.) and rapeseed (Brassica rapa L.). Root-thickness in the F2 plants showed a continuous distribution from 2.2-10.7 cm, suggesting the effects of a few major and minor genes. BSA, using three bulks based on root-thickness, suggested that an AFLP marker (EACCMCAG-220) which appeared only in plants with a thickened root, was linked to a locus that controlled root size. This AFLP band was cloned, sequenced, and successfully converted into a simple, dominant, sequence characterised amplified region (SCAR) marker. This marker may help to identify turnip genotypes with thicker taproots in segreating progenies of root crops during marker-assisted selection (MAS) breeding programmes.
机译：在来自芜菁（ Brassica rapa L）杂交后代的F 2 群体中，采用体积分离分离分析（BSA）鉴定了与控制贮藏根厚的基因座相关的DNA标记。 。ssp。 rapifera Matzg。和油菜籽（ Brassica rapa L.）。 F 2 植物的根厚在2.2-10.7 cm范围内呈连续分布，表明一些主要和次要基因的影响。 BSA使用了三个基于根厚的散装，建议将AFLP标记（E ACC M CAG -220）仅出现在根部增厚的植物中，到控制根大小的位置。将该AFLP带克隆，测序并成功转化为简单的，显性的，序列特征性的扩增区域（SCAR）标记。在标记辅助选择（MAS）育种计划中，该标记可能有助于鉴定根系作物的后代中主根较粗的萝卜基因型。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号