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首页> 外文期刊>The Journal of Horticultural Science & Biotechnology >Identification of S-haplotype-specific S-RNase and SFB alleles in native Chinese apricot (Prunus armeniaca L.).
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Identification of S-haplotype-specific S-RNase and SFB alleles in native Chinese apricot (Prunus armeniaca L.).

机译：天然杏中 S -单倍型特异性 S-RNase 和 SFB 等位基因的鉴定。 ）。

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摘要

Chinese apricot (Prunus armeniaca L.) shows gametophytic self-incompatibility (GSI) controlled by a single locus containing at least two linked genes [i.e., the S-RNase gene and the pollen-expressed SFB (or SLF) gene] as do other fruit species in the family, Rosaceae. To elucidate the S-genotypes of 14 native Chinese apricot cultivars, PCR was performed using primers designed from Prunus S-RNase and SFB consensus sequences. After cloning and sequencing the PCR products, the S-genotypes of all 14 apricot cultivars were determined, and eight new S-RNase alleles and nine SFB alleles were identified. The S-RNases shared typical structural features with S-RNases from other Prunus spp. exhibiting GSI. The SFB genes showed similar structural characteristics to SFB genes in other Prunus spp. The intron sequences of the SFB genes revealed sequence and length polymorphisms. The deduced level of amino acid sequence identity for the eight new S-RNase alleles was 66.4-100% in P. armeniaca, while the similarity of the SFB alleles was 73.7-98.6%. The physical distances between the SFB and S-RNase genes was determined exactly in the S9, S11, S17, and S26-haplotypes, confirming that the S-RNase and SFB genes were linked. The range of distances between the two genes was 299-1,061 bp. This study increases our knowledge on the S-genotypes of apricot native to China, and enriches our genomic information on GSI in the Prunus genus.
机译：中国杏（P.us armeniaca L.）表现出配子体自交不亲和性（GSI），其受单个基因座控制，该基因座包含至少两个连锁基因[即，S-RNase 基因和花粉表达的 SFB （或 SLF ）基因]，以及蔷薇科中的其他果实。为了阐明14个中国杏品种的 S 基因型，使用从李子S-RNase 和 SFB 共有序列设计的引物进行PCR。在对PCR产物进行克隆和测序后，确定了所有14个杏品种的 S 基因型，以及八个新的 S-RNase 等位基因和九个 SFB 确定了等位基因。 S-RNases 与其他李子属的 S-RNases 具有典型的结构特征。展示GSI。 SFB 基因显示出与其他 Prunus 菌种中 SFB 基因相似的结构特征。 SFB 基因的内含子序列揭示了序列和长度多态性。在 P中，八个新的 S-RNase 等位基因推导的氨基酸序列同一性水平为66.4-100％。亚美尼亚，而 SFB 等位基因的相似性为73.7-98.6％。 SFB 和 S-RNase 基因之间的物理距离是在 S 9 < / i>， S 11 ， S 17 和 S 26 -单倍型，确认 S-RNase 和 SFB 基因被链接。两个基因之间的距离范围是299-1,061 bp。这项研究增加了我们对中国原产杏的 S 基因型的了解，并丰富了我们对李属GSI的基因组信息。

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