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首页> 外文期刊>The Journal of Horticultural Science & Biotechnology >Transformation of Hibiscus rosa-sinensis L. by Agrobacterium rhizogenes.
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Transformation of Hibiscus rosa-sinensis L. by Agrobacterium rhizogenes.

机译：发根农杆菌对罗莎木槿的转化

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摘要

Transgenic hairy roots were induced from leaf, petiole, and shoot explants of in vitro-raised plantlets of potted Hibiscus rosa-sinensis L. 'Cassiopeia Wind Yellow' plants after co-cultivation with three different wild-type strains of Agrobacterium rhizogenes. The highest infection rate (56.4%) was obtained in explants inoculated with A. rhizogenes strain ATCC43057, followed by ATCC15834 (44.0%), and NCPPB8196 (34.4%). The highest infection rate (60.5%) was obtained using leaf basal segments, followed by petioles (44.0%), leaf tips (40.0%), and shoots (20%). A total of 102 putative transgenic root lines were produced. The hairy root lines did not grow without applying 0.2 mg l-1 indole-3-butryric acid (IBA) and 0.2 mg l-1 alpha -naphthaleneacetic acid (NAA). The presence of T-DNA was confirmed in six hairy root lines, and no A. rhizogenes contamination was detected by polymerase chain reaction.
机译：从盆栽的 Hiscus rosa-sinensis L.'Cassiopeia Wind Yellow'植物的共培养后的叶片，叶柄和芽的外植体诱导转基因毛状根。三种不同的发根农杆菌野生型菌株培养。接种iA的外植体获得最高的感染率（56.4％）。发根菌株ATCC43057，其次是ATCC15834（44.0％）和NCPPB8196（34.4％）。使用叶基部获得最高的感染率（60.5％），其次是叶柄（44.0％），叶尖（40.0％）和枝条（20％）。总共产生102个推定的转基因根系。如果不使用0.2 mg l -1 吲哚-3-丁酸（IBA）和0.2 mg l -1 α-萘乙酸（NAA），则毛状根系不会生长。 。在六个有毛的根系中证实了T-DNA的存在，没有A。通过聚合酶链反应检测发根根污染。

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