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首页> 外文期刊>The Journal of Horticultural Science & Biotechnology >Regeneration and proliferation of pathogen-free horseradish (Armoracia rusticana) plants in vitro.
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Regeneration and proliferation of pathogen-free horseradish (Armoracia rusticana) plants in vitro.

机译：无病原体辣根植物在体外的再生和增殖。

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摘要

Horseradish (Armoracia rusticana Gaertn.) leaf explants were tested for their ability to regenerate and proliferate shoots in vitro. Leaf explants were grown on Murashige and Skoog (MS) medium supplemented with different concentrations of either naphthalene acetic acid (NAA) or benzyladenine (BA), or a combination of both. The optimum number of regenerated shoots occurred at 15 micro M NAA, while the optimum number of proliferated shoots occurred at 2 micro M BA. Higher concentrations of BA resulted in reduced shoot height, inhibited rooting, and increased leaf deformation. A sample (1,000 plants) of both regenerated and proliferated plants was rooted, acclimatised to greenhouse conditions, then field-planted in two subsequent years. All plants survived. The ability of ten virus-free (VF) and ten virus-infected (VI) horseradish cultivars to regenerate shoots in vitro was tested. Nine of the ten VF cultivars regenerated more vigorous and healthier shoots than VI plants. The results of these experiments represent the first steps towards establishing a certified root programme to provide horseradish growers with large numbers of pathogen-free plants to restore yields of good quality roots.
机译：测试了辣根（ Armoracia Rustana Gaertn。）叶外植体在体外的再生和增殖能力。叶片外植体生长在Murashige和Skoog（MS）培养基上，该培养基添加了不同浓度的萘乙酸（NAA）或苄基腺嘌呤（BA）或两者的组合。再生芽的最佳数量出现在15 micro M NAA，而增殖芽的最佳数量出现在2 micro M BA。较高的BA浓度导致降低的苗高，抑制生根和增加叶片变形。将再生和增生植物的样品（1,000株植物）生根，使其适应温室条件，然后在随后的两年中进行大田种植。所有植物均存活。测试了十个无病毒（VF）和十个被病毒感染（VI）的辣根品种在体外再生芽的能力。与VI植物相比，十个VF品种中有九个再生的芽更健康。这些实验的结果代表着建立认证的根计划的第一步，该计划旨在为辣根种植者提供大量无病原体的植物，以恢复优质根的产量。

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