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首页> 外文期刊>The Journal of Horticultural Science & Biotechnology >Identification of 57 sweet orange cultivars using AFLP markers.
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Identification of 57 sweet orange cultivars using AFLP markers.

机译：使用AFLP标记鉴定57个甜橙品种。

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摘要

Sweet orange (Citrus sinensis) represents an important group of Citrus fruit; however, the identification of sweet orange cultivars during vegetative growth can be difficult. A study on the genetic identification of sweet orange cultivars may be significant for the sweet orange nursery industry, for cultivar-rights protection, and is important for the genetic evaluation and conservation of these orange cultivars. In this study, amplified fragment length polymorphism (AFLP) markers were used to genotype 57 sweet orange cultivars. Ten PCR primer pairs generated 629 unique AFLP bands, with a size range of 50-500 bp. Seventy-four bands (11.8%) were polymorphic. On average, each primer pair produced 62.9 fragments, with 7.4 polymorphic fragments. A dendrogram of the 57 sweet orange cultivars was constructed based on an UPGMA analysis using Jaccard's coefficients of similarity. This provided a clear comparison of the genetic variation between cultivars and an ability to identify them. From Jaccard's coefficients, 56 of the 57 cultivars examined were genetically close, with coefficient values >=0.985. 'Variegated Navel' was less closely related, with a much lower coefficient value (0.94). Among the 57 cultivars, 28 sub-groups, some consisting of only one cultivar, could be separated by their AFLP fingerprints. Compared to ISSR and SSR markers, AFLP seemed to be the preferential marker technique for the identification of sweet orange cultivars.
机译：甜橙（ Citrus sinensis ）代表了重要的 Citrus 水果类。然而，在营养生长过程中很难鉴定出甜橙品种。对甜橙品种的遗传鉴定研究对甜橙苗圃产业，对品种权利的保护具有重要意义，对这些甜橙品种的遗传评价和保存具有重要意义。在这项研究中，扩增的片段长度多态性（AFLP）标记用于基因型57甜橙品种。十对PCR引物对产生629条独特的AFLP条带，大小范围为50-500 bp。七个带（11.8％）是多态的。平均而言，每个引物对产生62.9个片段，具有7.4个多态性片段。基于雅克卡德相似系数的UPGMA分析，构建了57个甜橙品种的树状图。这清楚地比较了品种之间的遗传变异以及鉴定它们的能力。根据雅卡德系数，所研究的57个品种中有56个在遗传上接近，系数值> = 0.985。 “杂色肚脐”的相关性较低，系数值较低（0.94）。在57个品种中，有28个亚组，其中一些仅由一个品种组成，可以通过AFLP指纹图谱进行区分。与ISSR和SSR标记相比，AFLP似乎是鉴定甜橙品种的首选标记技术。

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