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首页> 外文期刊>The Asian and Australasian Journal of Plant Science and Biotechnology >Effects of Genotype, Plant Growth Regulators and Explant Source on Callus Induction in Cotton (Gossypium hirsutum L.)
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Effects of Genotype, Plant Growth Regulators and Explant Source on Callus Induction in Cotton (Gossypium hirsutum L.)

机译：基因型，植物生长调节剂和外源对棉花愈伤组织诱导的影响

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摘要

In this study callus induction of 5 elite upland cotton genotypes ('PIL-8', T 1378', T 1861', 'LH 1995' and 'LH 2076') were tested on five different callus induction (MS + 1.0 mg/l Kin + 1.5 mg/l IAA; MS + 2.0 mg/1 NAA + 0.1 mg/1 Kin; MSB (MS + Vit. B5) + 1.0 mg/l ZT + 1.0 mg/1 Kin + 560 mg/1 proline; MSB +2.5 mg/1 ZT + 1.0 mg/1 2,4-D; MS + 1.0 mg/1 2,4-D + 1.0 mg/1 Kin + 560 mg/l proline) and somatic embryo initiation-maturation media that were previously found to be capable of inducing somatic embryogenesis in cotton. All genotypes tested produced callus on different media within 4 to 6 weeks. Analysis of variance revealed significant differences in callus induction, days taken to callus induction and callus growth index in different explants, genotypes and media composition. Of the two explants compared, hypocotyls and cotyledons, the former were most responsive to callus induction and proliferation than the latter. Hypocotyls showed a significantly higher percentage of callusing ranging from 35.05 to 78.44% with an average of 59.45% while the corresponding values for cotyledons were 27.75 to 59.92% and 45%, respectively. PIL-8 recorded the highest percentage of callus induction (78.44%) and was the only genotype that induced callus within 30 days. Different types of media tested revealed that plant growth regulator type had a significant effect on callus induction and physical appearance. The callus induced on MS medium containing 2,4-D was brown and of low quality compared to that produced on MS media containing NAA, Kin and ZT combinations. However, medium containing Kin resulted in the formation of compact callus with a number of roots emerging from it. The results of this study will pave the way for establishing a future in vitro regeneration system and transformation methods for elite Indian cultivars.
机译：在这项研究中，测试了5种优良陆地棉基因型（'PIL-8'，T 1378'，T 1861'，'LH 1995'和'LH 2076'）的愈伤组织诱导，对五种不同的愈伤组织诱导进行了测试（MS + 1.0 mg / l Kin + 1.5 mg / l IAA; MS + 2.0 mg / 1 NAA + 0.1 mg / 1 Kin; MSB（MS + Vit.B5）+ 1.0 mg / l ZT + 1.0 mg / 1 Kin + 560 mg / 1 Proline; MSB +2.5 mg / 1 ZT + 1.0 mg / 1 2,4-D; MS + 1.0 mg / 1 2,4-D + 1.0 mg / 1 Kin + 560 mg / l脯氨酸）和体细胞胚胎起始成熟培养基以前发现能够诱导棉花的体细胞胚发生。测试的所有基因型在4至6周内在不同培养基上产生愈伤组织。方差分析显示在不同的外植体，基因型和培养基组成中，愈伤组织诱导，愈伤组织诱导所花费的天数和愈伤组织生长指数存在显着差异。在比较的两个外植体中，下胚轴和子叶中，前者对愈伤组织的诱导和增殖反应比后者更强。下胚轴显示出显着更高的愈伤组织百分比，范围从35.05至78.44％，平均为59.45％，而子叶的相应值分别为27.75至59.92％和45％。 PIL-8记录的愈伤组织诱导率最高（78.44％），并且是30天内唯一诱导愈伤组织的基因型。测试的不同类型的培养基表明，植物生长调节剂类型对愈伤组织的诱导和外观有显着影响。与在包含NAA，Kin和ZT组合的MS培养基上产生的愈伤组织相比，在包含2,4-D的MS培养基上诱导的愈伤组织呈褐色且质量较低。但是，含有Kin的培养基会形成致密的愈伤组织，并从中生出许多根。这项研究的结果将为建立未来印度优良品种的体外再生体系和转化方法铺平道路。

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