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首页> 外文期刊>Chinese journal of digestive diseases >Investigation of the sensitivities of distinct gastric cancer cells to parvovirus H-l induced cytotoxicity
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Investigation of the sensitivities of distinct gastric cancer cells to parvovirus H-l induced cytotoxicity

机译：不同胃癌细胞对细小病毒H-1诱导的细胞毒性的敏感性研究

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OBJECTIVE: To investigate the sensitivities of distinct gastric cancer cells to parvovims H-l induced cytotoxicity and the possible mechanism(s).METHODS: There were six distinct differentiated gastric cancer cell lines: HGC27 (undifferentiated), BGC823 (undifferentiated), MKN45 (poorly differentiated), AGS (poorly differentiated), SGC7901 (moderately differentiated) and MKN28 (well differentiated). The cell cycle distributions were measured by flow cytometry and the differential sensitivities of the six distinct gastric cancer cells after H-l virus infection were detected by MTT assay. RT-PCR was used to detect viral NS1 gene expression in all six gastric cancer cell lines.
机译：目的：研究不同胃癌细胞对细小病毒H1诱导的细胞毒性的敏感性及可能的作用机制。方法：共有六种不同的胃癌细胞系：HGC27（未分化），BGC823（未分化），MKN45（低分化）。 ），AGS（低分化），SGC7901（中分化）和MKN28（高分化）。通过流式细胞术测量细胞周期分布，并通过MTT测定法检测H-1病毒感染后六个不同胃癌细胞的差异敏感性。 RT-PCR被用于检测所有六个胃癌细胞系中病毒NS1基因的表达。

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