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首页> 外文期刊>Chinese journal of digestive diseases >Therapeutic vaccination against Helicobacter pylori infection with attenuated recombinant Salmonella typhimurium urease B subunit and catalase in mice
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Therapeutic vaccination against Helicobacter pylori infection with attenuated recombinant Salmonella typhimurium urease B subunit and catalase in mice

机译：减毒重组鼠伤寒沙门氏菌脲酶B亚单位和过氧化氢酶对幽门螺杆菌感染的治疗疫苗

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OBJECTIVE: To investigate the effects of oral immunization with attenuated recombinant Salmonella typhimurium urease B subunit and catalase vaccines in the treatment of Helicobacter pylori infection in a H. pylori infected mouse model.METHODS: Thirty C57BL/6 mice were randomized into three groups and challenged twice with oral administration of H. pylori within 3 days. Four weeks after the second challenge, the mice were immunized by oral administration of attenuated recombinant S. typhimurium urease B subunit (group A), attenuated recombinant S. typhimurium catalase (group B) or saline (group C), and all mice were killed 4 weeks later. The stomachs were collected for a rapid urease test, modified Giemsa staining and quantitative culture to observe the density of H. pylori, hematoxylin-eosin staining was performed to assess the presence of inflammation and lymphocytes from the spleen were used for the lymphoproliferation assay.
机译：目的：研究减毒重组鼠伤寒沙门氏菌脲酶B亚单位和过氧化氢酶疫苗的口服免疫在幽门螺杆菌感染小鼠模型中治疗幽门螺杆菌感染的方法。方法：将30只C57BL / 6小鼠随机分为三组并进行攻击在3天内口服两次幽门螺杆菌。第二次攻击后四周，通过口服减毒重组鼠伤寒沙门氏菌脲酶B亚基（A组），减毒重组鼠伤寒沙门氏菌过氧化氢酶（B组）或生理盐水（C组）免疫小鼠，并杀死所有小鼠。 4周后。收集胃进行快速尿素酶测试，改良的吉姆萨（Giemsa）染色和定量培养以观察幽门螺杆菌的密度，苏木精-伊红染色进行评估以评估炎症的存在，并将脾脏中的淋巴细胞用于淋巴细胞增殖试验。

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