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首页> 外文期刊>Chinese journal of digestive diseases >Epidemiology and genotypes of HEV in Wuhan.
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Epidemiology and genotypes of HEV in Wuhan.

机译：武汉市戊型肝炎的流行病学和基因型。

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BACKGROUND: Understanding the genotype and clinical features of the hepatitis E virus (HEV) are important for understanding its characteristics, for evaluating region-specific diagnostic assays, and producing vaccines. OBJECTIVES: To investigate the epidemiology and the genotypes of HEV among outpatients and inpatients in the Department of Infectious Diseases of Tongji Hospital in Wuhan, China. METHODS: Clinical data were elicited from the hospital records of patients who were clinically diagnosed with acute hepatitis between January 2000 and August 2004 (4920 patients). Of these cases, 120 patients with anti-HEV-IgM, IgG-positive were selected to analysis. Conserved genomic sequences of open reading frame 2 (345 bp) in the HEV gene were detected using polymerase chain reaction, 25 of which were cloned and sequenced. Clustal X and Mega software were used for phylogenetic analysis of genotypes strains. RESULTS: The HEV infection rate is gradually increasing in Wuhan. The number of male patients was 3.3-fold greater than the number of female patients found in clinical investigations. People aged 30-59 years are more susceptible to infection, and people are more susceptible in March-June. Twenty-five isolates shared the same genotype, genotype IV, with 82.61-98.55% nucleotide identity. This genotype had 76.52-81.74%, 70.43-73.04%, 76.52-81.16%, and 84.35-88.70% homology with the nucleotide sequence of HEV genotypes I-IV, respectively. Phylogenetic analysis suggested that these 25 isolates represented at least three different subtypes, but there were no significant differences found in the epidemiological features or liver function of patients with the three subtypes. CONCLUSIONS: HEV sequences isolated from patients in Wuhan belong to different subtypes of HEV genotype IV.
机译：背景：了解戊型肝炎病毒（HEV）的基因型和临床特征对于理解其特征，评估区域特异性诊断方法和生产疫苗非常重要。目的：调查武汉市同济医院传染病科门诊和住院患者HEV的流行病学和基因型。方法：从2000年1月至2004年8月期间临床诊断为急性肝炎的患者（4920例患者）的医院记录中获取临床数据。在这些病例中，选择了120例抗HEV-IgM，IgG阳性的患者进行分析。使用聚合酶链反应检测HEV基因中开放阅读框2（345 bp）的保守基因组序列，其中25个被克隆并测序。 Clustal X和Mega软件用于基因型菌株的系统发育分析。结果：武汉市戊型肝炎病毒感染率正在逐步增加。在临床研究中发现的男性患者人数比女性患者人数高3.3倍。 30-59岁的人更容易受到感染，3月至6月的人更容易受到感染。 25个分离株具有相同的基因型，即基因型IV，具有82.61-98.55％的核苷酸同一性。该基因型与HEV基因型I-IV的核苷酸序列分别具有76.52-81.74％，70.43-73.04％，76.52-81.16％和84.35-88.70％同源性。系统发育分析表明，这25个分离株代表至少三种不同的亚型，但是在这三种亚型的患者的流行病学特征或肝功能方面没有发现显着差异。结论：从武汉患者中分离出的HEV序列属于HEV基因型IV的不同亚型。

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