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首页> 外文期刊>The Journal of Horticultural Science & Biotechnology >Genetic diversity and phylogeny of tea plant (Camellia sinensis) and its related species and varieties in the section Thea genus Camellia determined by randomly amplified polymorphic DNA analysis
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Genetic diversity and phylogeny of tea plant (Camellia sinensis) and its related species and varieties in the section Thea genus Camellia determined by randomly amplified polymorphic DNA analysis

机译：用随机扩增多态性DNA分析法确定茶树茶树部分茶树及其近缘种和品种的遗传多样性，系统发育

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摘要

The genetic diversity and molecular phylogeny of tea plant (C. sinensis) and its 23 related species and varieties were investigated by randomly amplified polymorphic DNA (RAPD) analysis. Fifteen decamer oligonucleotide primers were selected from the61 screened. A total of 102 polymorphic bands (6.8 diversity/primer) out of 107 reproducible products (7.1 fragments per primer) were amplified from the selected 15 primers, corresponding to 95.3% diversity of the amplification bands. The molecular phylogenetic dendrogram of section Thea was constructed using UPGMA and basing on Nei and Li's similarity coefficient from pairwise comparisons of RAPD data between 24 species and varieties. According to the dendrogram, there were two groups, corresponding to 3- and 5-locule ovary in morphology. There were two and three subgroups in each group, respectively. The genetic relationship and the molecular phylogeny among section Thea were discussed. RAPD not only confirmed a large body of morphological classification and evolution of section Thea of the genus Camellia proposed in previous work, but also provided novel and robust evidence of the genetic diversity and phylogeny of the tea plant and its related species.
机译：通过随机扩增多态性DNA（RAPD）分析研究了茶树（C. sinensis）及其23个相关物种和品种的遗传多样性和分子系统发育。从所筛选的61种中选择了十五种十聚体寡核苷酸引物。从选定的15个引物中扩增出107个可复制产物中的102条多态性条带（6.8多样性/引物）（每个引物7.1片段），对应于95.3％的扩增条带。 Thea的分子系统进化树是使用UPGMA并基于Nei和Li的相似系数，通过对24个物种和变种之间的RAPD数据进行成对比较而构建的。根据树状图，有两组，在形态上对应于3和5位子房。每个组中分别有两个和三个子组。讨论了Thea区之间的亲缘关系和分子系统发育。 RAPD不仅证实了先前工作中提出的茶花属Thea部分的大部分形态学分类和进化，而且为茶树及其近缘种的遗传多样性和系统发育提供了新颖而有力的证据。

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