...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Chinese journal of digestive diseases >Tripterygium glycosides suppress production of interleukin-4 by splenocytes in oxazolone-induced murine colitis
【24h】

Tripterygium glycosides suppress production of interleukin-4 by splenocytes in oxazolone-induced murine colitis

机译：雷公藤多甙抑制恶唑酮诱导的小鼠结肠炎中脾细胞产生白介素-4。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

To investigate the in vitro effect of Tripterygium glycosides (TG) on cytokine production by splenocytes in oxazolone (OXZ)-induced colitis in mice.Oxazolone (6 mg in 50% ethanol) was administered to male SJL/J mice intrarectally to induce colitis and the mice were killed 3 days later. Isolated splenocytes were cultured for 24 h in the presence of phorbol myristate acetate and ionomycin. A preparation of Tripterygium glycosides at a concentration of either 0.1 mg/mL or 0.01 mg/mL was added to the culture medium of splenocytes. Production of interferon gamma (IFN-y) and interleukin-4 (IL-4) in the supernatant were measured by ELISA.
机译：为了研究雷公藤多甙（TG）对恶唑酮（OXZ）诱导的结肠炎小鼠脾细胞产生细胞因子的体外作用，向雄性SJL / J小鼠直肠内施用恶唑酮（6 mg，50％乙醇）诱导结肠炎和3天后将小鼠杀死。分离的脾细胞在佛波醇肉豆蔻酸酯乙酸盐和离子霉素的存在下培养24小时。将浓度为0.1 mg / mL或0.01 mg / mL的雷公藤多甙制剂添加到脾细胞培养基中。通过ELISA测量上清液中干扰素γ（IFN-γ）和白介素4（IL-4）的产生。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号