Cloning and characterization of human Lnk, an adaptor protein with pleckstrin homology and Src homology 2 domains that can inhibit T cell activation.

Li Y; He X; Schembri King-J; Jakes S; Hayashi J

首页> 外文期刊>The Journal of Immunology: Official Journal of the American Association of Immunologists >Cloning and characterization of human Lnk, an adaptor protein with pleckstrin homology and Src homology 2 domains that can inhibit T cell activation.

【24h】

Cloning and characterization of human Lnk, an adaptor protein with pleckstrin homology and Src homology 2 domains that can inhibit T cell activation.

机译：人Lnk的克隆和表征，Lnk是一种具有pleckstrin同源性和Src同源性2结构域的衔接蛋白，可以抑制T细胞活化。

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Lnk was originally cloned from a rat lymph node cDNA library and shown to participate in T cell signaling. Human Lnk (hLnk) was cloned by screening a Jurkat cell cDNA library. hLnk has a calculated molecular mass of 63 kDa, and its deduced amino acid sequence indicates the presence of an N-terminal proline-rich region, a pleckstrin homology domain, and a Src homology 2 domain. When expressed in COS cells, hLnk migrates with an apparent molecular mass of 75 kDa. Confocal fluorescence microscope analysis indicates that in COS cells transfected with an expression vector encoding a chimeric Lnk-green fluorescent protein, hLnk is found at the juxtanuclear compartment and also appears to be localized at the plasma membrane. Lnk is tyrosine-phosphorylated by p56lck. Following phosphorylation, p56lck binds to tyrosine-phosphorylated hLnk through its Src homology 2 domain. In COS cells cotransfected with hLnk, p56lck, and CD8-zeta, hLnk associated with tyrosine-phosphorylated TCR zeta-chain through its Src homology 2 domain. The overexpression of Lnk in Jurkat cells led to an inhibition of anti-CD3 mediated NF-AT-Luc activation. Our study reveals a potentially new mechanism of T cell-negative regulation.

机译：Lnk最初是从大鼠淋巴结cDNA文库中克隆的，并显示参与T细胞信号转导。通过筛选Jurkat细胞cDNA文库克隆了人Lnk（hLnk）。 hLnk的计算分子量为63 kDa，其推导的氨基酸序列表明存在N末端富含脯氨酸的区域，pleckstrin同源结构域和Src同源2结构域。当在COS细胞中表达时，hLnk以75 kDa的表观分子量迁移。共聚焦荧光显微镜分析表明，在用编码嵌合的Lnk-绿色荧光蛋白的表达载体转染的COS细胞中，hLnk存在于近核室，并且似乎也位于质膜。 Lnk被p56lck酪氨酸磷酸化。磷酸化后，p56lck通过其Src同源2域与酪氨酸磷酸化的hLnk结合。在用hLnk，p56lck和CD8-zeta共转染的COS细胞中，hLnk通过其Src同源性2结构域与酪氨酸磷酸化的TCR zeta链相关。 Lnk在Jurkat细胞中的过度表达导致抑制抗CD3介导的NF-AT-Luc活化。我们的研究揭示了T细胞阴性调节的潜在新机制。

著录项

来源
《The Journal of Immunology: Official Journal of the American Association of Immunologists》 |2000年第10期|共8页
作者
Li Y; He X; Schembri King-J; Jakes S; Hayashi J;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类医学免疫学;免疫遗传学;
关键词
src Homology Domains; Blood Proteins; Lymphocyte Transformation; Phosphoproteins; src同源域; 血蛋白质类; 淋巴细胞转化; 磷蛋白类; 序列同源性; 氨基酸; T淋巴细胞;

机译：src Homology Domains;Blood Proteins;Lymphocyte Transformation;Phosphoproteins;src同源域;血蛋白质类;淋巴细胞转化;磷蛋白类;序列同源性;氨基酸;T淋巴细胞;

相似文献

外文文献
中文文献
专利

1. Cloning and characterization of human Lnk, an adaptor protein with pleckstrin homology and Src homology 2 domains that can inhibit T cell activation. [J] . Li Y, He X, Schembri King-J, The Journal of Immunology: Official Journal of the American Association of Immunologists . 2000,第10期

机译：人Lnk的克隆和表征，Lnk是一种具有pleckstrin同源性和Src同源性2结构域的衔接蛋白，可以抑制T细胞活化。
2. Adapter protein with a pleckstrin homology (PH) and an Src homology 2 (SH2) domain (APS) and SH2-B enhance insulin-receptor autophosphorylation, extracellular-signal-regulated kinase and phosphoinositide 3-kinase-dependent signalling [J] . Ahmed Z., Pillay TS. The Biochemical Journal . 2003,第Pta2期

机译：具有pleckstrin同源性（PH）和Src同源性2（SH2）域（APS）和SH2-B的衔接蛋白可增强胰岛素受体自磷酸化，细胞外信号调节激酶和磷酸肌醇3激酶依赖性信号传导
3. Adapter protein with a pleckstrin homology (PH) and an Src homology 2 (SH2) domain (APS) and SH2-B enhance insulin-receptor autophosphorylation, extracellular-signal-regulated kinase and phosphoinositide 3-kinase-dependent signalling [J] . Tahir S. PILLAY, Zamal AHMED The biochemical journal . 2003,第2期

机译：具有pleckstrin同源性（PH）和Src同源性2（SH2）域（APS）和SH2-B的衔接蛋白可增强胰岛素受体自磷酸化，细胞外信号调节激酶和磷酸肌醇3激酶依赖性信号传导
4. 3D-QSAR Modeling, Molecular Docking and Quantum Mechanical Approaches to Identify Pleckstrin Homology Domain of New AKT1 Inhibitors [C] . Rengarajan Kavitha, Chandrasekaran Meganathan National Conference on Hierarchically Structured Materials . 2019

机译：3D-QSAR建模，分子对接和量子力学方法，用于鉴定新AKT1抑制剂的Pleckstrin同源域
5. Regulation of PDK1 protein kinase activation by its C-terminal pleckstrin homology domain. [D] . Al-Ali, Hassan. 2010

机译：通过其C末端pleckstrin同源结构域调节PDK1蛋白激酶激活。
6. Adapter protein with a pleckstrin homology (PH) and an Src homology 2 (SH2) domain (APS) and SH2-B enhance insulin-receptor autophosphorylation extracellular-signal-regulated kinase and phosphoinositide 3-kinase-dependent signalling. [O] . Zamal Ahmed, Tahir S Pillay 2003

机译：具有pleckstrin同源性（PH）和Src同源性2（SH2）域（APS）和SH2-B的衔接蛋白可增强胰岛素受体的自身磷酸化细胞外信号调节的激酶和磷酸肌醇3激酶依赖性信号传导。
7. APS, an adaptor protein containing Pleckstrin homology (PH) and Src homology-2 (SH2) domains inhibits the JAK-STAT pathway in collaboration with c-Cbl [O] . T Wakioka, A Sasaki, K Mitsui, 1999

机译：APS，含有Pleckstrin同源性（pH）和SRC同源性-2（SH2）结构域的适配器蛋白质与C-CBL合作抑制Jak-Stat途径

Cloning and characterization of human Lnk, an adaptor protein with pleckstrin homology and Src homology 2 domains that can inhibit T cell activation.

摘要

著录项

相似文献

相关主题

期刊订阅