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首页> 外文期刊>The Journal of Immunology: Official Journal of the American Association of Immunologists >Human fibroblasts express functional IL-2 receptors formed by the IL-2R alpha- and beta-chain subunits: association of IL-2 binding with secretion of the monocyte chemoattractant protein-1.
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Human fibroblasts express functional IL-2 receptors formed by the IL-2R alpha- and beta-chain subunits: association of IL-2 binding with secretion of the monocyte chemoattractant protein-1.

机译：人类成纤维细胞表达由IL-2Rα-链和β-链亚基形成的功能性IL-2受体：IL-2结合与单核细胞趋化蛋白1分泌的关联。

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摘要

Expression of IL-2R was examined on human fibroblasts isolated from different tissues. By specific binding assay it is shown that [125I]IL-2 bound to subconfluent adult bone marrow and embryonic skin and lung fibroblasts. The presence of binding sites for IL-2 was also confirmed by immunofluorescence and flow cytometry analysis using mAbs specific for the p55 IL-2R alpha (anti-CD25), p75 IL-2R beta, and p64 IL-2R gamma subunits. Fibroblasts also constitutively transcribed the genes coding for IL-2R alpha and IL-2R beta and accumulated their respective mRNAs but failed to exhibit the IL-2R gamma-chain on the mRNA and protein level. Although addition of IL-2 to fibroblast cultures did not significantly alter growth kinetics of these cells, the IL-2R complex displayed by fibroblasts appeared to be functional in that addition of IL-2 to these cells led to enhanced expression of the JE gene coding for the monocyte chemoattractant protein-1 (MCP-1). Enhancement of fibroblast MCP-1/JE gene expression by IL-2 appeared to result from delayed MCP-1/JE mRNA decay rather than as a consequence of an acceleration of the MCP-1/JE gene transcription rate. IL-2 had, however, no effect on the expression of other cytokine genes including IL-1, IL-5, IL-6, IL-7, IL-8, IL-9, granulocyte-macrophage-CSF, macrophage-CSF or TNF. These observations suggest that the range of cellular targets of IL-2 is broader than originally appreciated. IL-2 may thus serve to integrate fibroblasts and monocytes into a coordinated response of the connective tissue initiated by T lymphocytes.
机译：在从不同组织分离的人成纤维细胞上检查了IL-2R的表达。通过特异性结合测定法显示[125I] IL-2与亚汇合的成年骨髓以及胚胎皮肤和肺成纤维细胞结合。 IL-2结合位点的存在也通过免疫荧光和流式细胞仪分析确定，使用对p55 IL-2Rα（抗CD25），p75 IL-2R beta和p64 IL-2Rγ亚基具有特异性的mAb。成纤维细胞还组成性地转录编码IL-2R alpha和IL-2R beta的基因，并积累其各自的mRNA，但在mRNA和蛋白质水平上未表现出IL-2Rγ链。尽管在成纤维细胞培养物中添加IL-2不会显着改变这些细胞的生长动力学，但成纤维细胞展示的IL-2R复合物似乎具有功能性，因为在这些细胞中添加IL-2导致增强的JE基因编码表达用于单核细胞趋化蛋白-1（MCP-1）。 IL-2增强成纤维细胞MCP-1 / JE基因表达似乎是由于MCP-1 / JE mRNA延迟延迟所致，而不是由于MCP-1 / JE基因转录速率加快所致。但是，IL-2对其他细胞因子基因的表达没有影响，包括IL-1，IL-5，IL-6，IL-7，IL-8，IL-9，粒细胞巨噬细胞-CSF，巨噬细胞-CSF或TNF。这些观察结果表明IL-2的细胞靶标的范围比最初预期的要宽。因此，IL-2可用于将成纤维细胞和单核细胞整合到由T淋巴细胞引发的结缔组织的协调反应中。

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