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首页> 外文期刊>The Journal of Immunology: Official Journal of the American Association of Immunologists >Expression screening of a yeast artificial chromosome contig refines the location of the mouse H3a minor histocompatibility antigen gene.
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Expression screening of a yeast artificial chromosome contig refines the location of the mouse H3a minor histocompatibility antigen gene.

机译：酵母人工染色体重叠群的表达筛选完善了小鼠H3a次要组织相容性抗原基因的位置。

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摘要

The H3 complex, on mouse Chromosome 2, is an important model locus for understanding mechanisms underlying non-self Ag recognition during tissue transplantation rejection between MHC-matched mouse strains. H3a is a minor histocompatibility Ag gene, located within H3, that encodes a polymorphic peptide alloantigen recognized by cytolytic T cells. Other genes within the complex include beta2-microglobulin and H3b. A yeast artificial chromosome (YAC) contig is described that spans the interval between D2Mit444 and D2Mit17, a region known to contain H3a. This contig refines the position of many genes and anonymous loci. In addition, 23 new sequence-tagged sites are described that further increase the genetic resolution surrounding H3a. A novel assay was developed to determine the location of H3a within the contig. Representative YACs were modified by retrofitting with a mammalian selectable marker, and then introduced by spheroplast fusion into mouse L cells. YAC-containing L cells were screened for the expression of the YAC-encoded H3a(a) Ag by using them as targets in a cell-mediated lympholysis assay with H3a(a)-specific CTLs. A single YAC carrying H3a was identified. Based on the location of this YAC within the contig, many candidate genes can be eliminated. The data position H3a between Tyro3 and Epb4.2, in close proximity to Capn3. These studies illustrate how genetic and genomic information can be exploited toward identifying genes encoding not only histocompatibility Ags, but also any autoantigen recognized by T cells.
机译：小鼠2号染色体上的H3复合体是一个重要的模型位点，用于了解MHC匹配的小鼠品系之间组织移植排斥过程中非自我Ag识别的潜在机制。 H3a是位于H3内的次要组织相容性Ag基因，其编码溶细胞性T细胞识别的多态肽同种抗原。复合物中的其他基因包括β2-微球蛋白和H3b。描述了跨越D2Mit444和D2Mit17（已知包含H3a的区域）之间的间隔的酵母人工染色体（YAC）重叠群。该重叠群完善了许多基因和匿名基因座的位置。此外，描述了23个新的序列标记位点，这些位点进一步提高了H3a周围的遗传分辨率。开发了一种新颖的测定法来确定H3a在重叠群内的位置。通过用哺乳动物选择标记改造来修饰代表性的YAC，然后通过原生质球融合将其引入小鼠L细胞。通过在H3a（a）特异性CTL介导的细胞介导的淋巴测定中将YAC编码的H3a（a）Ag用作靶标，筛选包含YAC的L细胞的表达。鉴定出携带H3a的单个YAC。基于该YAC在重叠群内的位置，可以消除许多候选基因。 Tyro3和Epb4.2之间的数据位置H3a，紧邻Capn3。这些研究说明了如何利用遗传和基因组信息来鉴定不仅编码组织相容性Ags的基因，而且还能鉴定T细胞识别的任何自身抗原的基因。

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