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首页> 外文期刊>The Journal of Infectious Diseases >Increased plasma interleukin-7 level correlates with decreased CD127 and Increased CD132 extracellular expression on T cell subsets in patients with HIV-1 infection.
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Increased plasma interleukin-7 level correlates with decreased CD127 and Increased CD132 extracellular expression on T cell subsets in patients with HIV-1 infection.

机译：血浆白细胞介素7水平升高与HIV-1感染患者T细胞亚群上CD127减少和CD132细胞外表达增加有关。

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BACKGROUND: Interleukin (IL)-7 levels are increased in patients with human immunodeficiency virus type 1 (HIV-1)-associated lymphopenia; however, the effects of this on IL-7 receptor (IL-7R) expression, disease progression, and immune reconstitution remain unclear. METHODS: Plasma IL-7 levels were measured, by enzyme-linked immunoassay, in patients with primary, chronic, or long-term nonprogressive HIV-1 infection (PHI, CHI, and LTNP, respectively) before and after 40-48 weeks of antiretroviral therapy (ART). Cell-surface expression and intracellular expression of the IL-7R components CD127 and CD132 were measured by flow cytometry. The effects of IL-7 and cycloheximide on IL-7R expression by peripheral blood mononuclear cells were examined in vitro. RESULTS: Plasma IL-7 levels were increased in both patients with PHI and those with CHI; administration of ART resulted in normalized plasma IL-7 levels in patients with PHI but not in those with CHI. Plasma IL-7 levels positively correlated with CD4(+) T cell immune reconstitution in patients with PHI. In vitro, exogenous IL-7 rapidly down-regulated cell-surface CD127 expression, but not CD132 expression, whereas subsequent reexpression required active protein synthesis. HIV-1 infection resulted in progressive decreases in the CD127(+)132(-) subset and increases in the CD127(-)132(+) subset of CD4(+) and CD8(+) T cells. Changes in CD4(+) T cell expression of IL-7R components were evident in patients with LTNP who lost viral control, and these changes preceded increases in plasma IL-7 levels. CONCLUSIONS: Perturbations in the IL-7/IL-7R system were clearly associated with disease progression but did not reliably predict immune reconstitution.
机译：背景：人类免疫缺陷病毒1型（HIV-1）相关的淋巴细胞减少症患者的白细胞介素（IL）-7水平升高。然而，其对IL-7受体（IL-7R）表达，疾病进展和免疫重建的影响仍不清楚。方法：通过酶联免疫测定法，在40-48周的原发，慢性或长期非进行性HIV-1感染（分别为PHI，CHI和LTNP）患者中测定血浆IL-7水平。抗逆转录病毒疗法（ART）。通过流式细胞术测量IL-7R组分CD127和CD132的细胞表面表达和细胞内表达。体外检查了IL-7和环己酰亚胺对外周血单核细胞IL-7R表达的影响。结果：PHI和CHI患者血浆IL-7水平均升高。在PHI患者中，ART的使用可使血浆IL-7水平达到正常水平，而CHI患者则未达到这一水平。 PHI患者的血浆IL-7水平与CD4（+）T细胞免疫重建呈正相关。在体外，外源性IL-7迅速下调细胞表面CD127的表达，但不下调CD132的表达，而随后的重新表达则需要活性蛋白的合成。 HIV-1感染导致CD4（+）和CD8（+）T细胞的CD127（+）132（-）子集逐渐减少，而CD127（-）132（+）子集增加。在失去病毒控制的LTNP患者中，IL-7R成分的CD4（+）T细胞表达发生了明显变化，这些变化先于血浆IL-7水平的升高。结论：IL-7 / IL-7R系统的紊乱显然与疾病进展有关，但不能可靠地预测免疫重建。

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