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首页> 外文期刊>The Journal of Infectious Diseases >Validation of immunoglobulin G enzyme-linked immunosorbent assay for antibodies to pneumococcal surface adhesin A in the diagnosis of pneumococcal pneumonia among adults in Kenya.
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Validation of immunoglobulin G enzyme-linked immunosorbent assay for antibodies to pneumococcal surface adhesin A in the diagnosis of pneumococcal pneumonia among adults in Kenya.

机译：在肯尼亚成年人中诊断肺炎球菌肺炎的免疫球蛋白G酶联免疫吸附法对肺炎球菌表面粘附素A抗体的验证。

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摘要

Epidemiologic studies of pneumococcal pneumonia, including vaccine efficacy trials, are hampered by a lack of sensitive and specific diagnostic tests. Pneumococcal surface adhesin A (PsaA) is a genetically conserved, surface-expressed protein common to all serotypes of Streptococcus pneumoniae and is highly immunogenic. Detection of anti-PsaA immunoglobulin G by recombinant PsaA enzyme-linked immunosorbent assay was evaluated for diagnosis of pneumococcal pneumonia in paired serum samples from 4 adult populations: 47 healthy control subjects, 56 clinic control subjects without pneumococcal disease syndromes, 109 patients with pneumococcal pneumonia, and 93 pneumonia patients with no evidence of pneumococcal etiology. By considering a 2-fold increase in antibody concentration as positive, sensitivity was 0.70, and specificity was 0.98. With a 1.3-fold increase, these were 0.89 and 0.98, respectively. The test's performance was not affected by the patients' human immunodeficiency virus status or by the pneumococcal serotype. The combination of high sensitivity and high specificity makes this an ideal assay for epidemiologic studies of pneumococcal pneumonia.
机译：缺乏敏感性和特异性的诊断测试阻碍了肺炎球菌性肺炎的流行病学研究，包括疫苗功效试验。肺炎球菌表面粘附素A（PsaA）是一种遗传上保守的，在表面表达的蛋白质，对于肺炎链球菌的所有血清型均具有这种蛋白质，并且具有高度免疫原性。评估了通过重组PsaA酶联免疫吸附测定法检测抗PsaA免疫球蛋白G的诊断，以诊断来自4个成人人群的配对血清样本中的肺炎球菌肺炎：47名健康对照组，56名无肺炎球菌综合症的临床对照组，109例肺炎球菌性肺炎，还有93名没有肺炎球菌病因学证据的肺炎患者。通过将抗体浓度增加2倍视为阳性，灵敏度为0.70，特异性为0.98。增长1.3倍，分别为0.89和0.98。该测试的性能不受患者人体免疫缺陷病毒状况或肺炎球菌血清型的影响。高灵敏度和高特异性相结合，使它成为肺炎球菌肺炎流行病学研究的理想方法。

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