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首页> 外文期刊>The Journal of investigative dermatology. >Stratum Corneum Keratin Structure, Function, and Formation: The Cubic Rod-Packing and Membrane Templating Model.
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Stratum Corneum Keratin Structure, Function, and Formation: The Cubic Rod-Packing and Membrane Templating Model.

机译：角质层角蛋白的结构，功能和形成：立方棒堆积和膜模板模型。

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摘要

A new model for stratum corneum keratin structure, function, and formation is presented. The structural and functional part of the model, which hereafter is referred to as "the cubic rod-packing model", postulates that stratum corneum keratin intermediate filaments are arranged according to a cubic-like rod-packing symmetry with or without the presence of an intracellular lipid membrane with cubic-like symmetry enveloping each individual filament. The new model could account for (i) the cryo-electron density pattern of the native corneocyte keratin matrix, (ii) the X-ray diffraction patterns, (iii) the swelling behavior, and (iv) the mechanical properties of mammalian stratum corneum. The morphogenetic part of the model, which hereafter is referred to as "the membrane templating model", postulates the presence in cellular space of a highly dynamic small lattice parameter (<30 nm) membrane structure with cubic-like symmetry, to which keratin is associated. It further proposes that membrane templating, rather than spontaneous self-assembly, is responsible for keratin intermediate filament formation and dynamics. The new model could account for (i) the cryo-electron density patterns of the native keratinocyte cytoplasmic space, (ii) the characteristic features of the keratin network formation process, (iii) the dynamic properties of keratin intermediate filaments, (iv) the close lipid association of keratin, (v) the insolubility in non-denaturating buffers and pronounced polymorphism of keratin assembled in vitro, and (vi) the measured reduction in cell volume and hydration level between the stratum granulosum and stratum corneum. Further, using cryo-transmission electron microscopy on native, fully hydrated, vitreous epidermis we show that the subfilametous keratin electron density pattern consists, both in corneocytes and in viable keratinocytes, of one axial subfilament surrounded by an undetermined number of peripheral subfilaments forming filaments with a diameter of approximately 8 nm.
机译：提出了角质层角蛋白结构，功能和形成的新模型。该模型的结构和功能部分（以下称为“立方棒堆积模型”）假定，角质层角蛋白中间丝是根据立方样的棒堆积对称性排列的，无论是否存在细胞内脂质膜呈立方状对称，包裹着每根细丝。新模型可以解释（i）天然角质细胞角蛋白基质的冷冻电子密度模式，（ii）X射线衍射图，（iii）溶胀行为和（iv）哺乳动物角质层的机械性能。该模型的形态发生部分（以下称为“膜模板模型”）假定细胞空间中存在具有立方样对称性的高动态小晶格参数（<30 nm）膜结构，角蛋白是相关。进一步提出，膜模板而不是自发的自组装负责角蛋白中间丝的形成和动力学。新模型可以解释（i）天然角质形成细胞胞质空间的低温电子密度模式，（ii）角蛋白网络形成过程的特征，（iii）角蛋白中间丝的动态特性，（iv）角蛋白紧密的脂质缔合，（v）在体外组装的非变性缓冲液中的不溶性和明显的角蛋白多态性，以及（vi）测得的颗粒层​​和角质层之间的细胞体积和水合水平降低。此外，在天然的，完全水合的玻璃状表皮上使用低温透射电子显微镜，我们发现丝状亚细胞角蛋白电子密度模式在角质细胞和存活的角质形成细胞中均由一个轴向亚丝围绕，该轴向亚丝被数目不定的周围亚丝所包围，形成细丝。直径约为8 nm。

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