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首页> 外文期刊>The Journal of investigative dermatology. >Endothelial cell surface alkaline phosphatase activity is induced by IL-6 released during wound repair.
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Endothelial cell surface alkaline phosphatase activity is induced by IL-6 released during wound repair.

机译：伤口修复过程中释放的IL-6诱导内皮细胞表面碱性磷酸酶活性。

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摘要

Phosphatase activity on endothelial cell surfaces is responsible, in part, for the conversion of adenosine nucleotides to adenosine, a potent vasodilator and anti-inflammatory mediator that can protect tissues from the ischemic damage that results from injury. To evaluate whether phosphatases are actively induced by a soluble factor released following injury, the effect of tissue fluids collected from porcine or human skin wounds was tested on primary cultures of endothelial cells. Phosphatase activity increased approximately 50-fold following 48-h culture in the presence of wound fluid. Inductive activity was present only in fluids collected during the inflammatory phase of wound repair. The phosphatase activity metabolized adenosine monophosphate to free phosphate and was the liver/bone/kidney alkaline phosphatase isoenzyme: activity was temperature- and levamisole-sensitive, 1-phenylalanine-resistant, and linked to the cell surface via phospholipid, and migrated at a size identical to this isozyme. interleukin-6 was identified as the phosphatase-inducing factor in wound fluid and the related cytokines, leukaemia inhibiting factor, and oncostatin M, caused a similar degree of alkaline phosphatase induction. Therefore, following injury, accumulation of interleukin-6 can lead to production by alkaline phosphatase of adenosine and subsequent protection from ischemic injury.
机译：内皮细胞表面的磷酸酶活性部分负责腺苷核苷酸向腺苷的转化，腺苷是一种有效的血管扩张剂和抗炎介质，可以保护组织免受损伤引起的缺血性损伤。为了评估磷酸酶是否被损伤后释放的可溶性因子有效地诱导，在内皮细胞的原代培养物中测试了从猪或人皮肤伤口收集的组织液的作用。在伤口液存在下培养48小时后，磷酸酶活性增加了约50倍。诱导活性仅在伤口修复的炎症阶段收集的液体中存在。磷酸酶活性将腺苷一磷酸代谢为游离磷酸，是肝脏/骨骼/肾脏碱性磷酸酶同工酶：活性对温度和左旋咪唑敏感，对1-苯丙氨酸敏感，并通过磷脂连接到细胞表面，并迁移至一定大小与此同工酶相同。白细胞介素6被确定为伤口液中的磷酸酶诱导因子，相关的细胞因子，白血病抑制因子和抑瘤素M引起了类似程度的碱性磷酸酶诱导。因此，在损伤后，白细胞介素6的积累可导致腺苷的碱性磷酸酶产生并随后保护其免受缺血性损伤。

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