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首页> 外文期刊>The Journal of investigative dermatology. >Ahnak/Desmoyokin Is Dispensable for Proliferation, Differentiation, and Maintenance of Integrity in Mouse Epidermis.
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Ahnak/Desmoyokin Is Dispensable for Proliferation, Differentiation, and Maintenance of Integrity in Mouse Epidermis.

机译：Ahnak / Desmoyokin对于小鼠表皮的增殖，分化和完整性保持是不可缺少的。

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摘要

Desmoyokin was first isolated from bovine muzzle epidermis and thought to be an epidermal desmosome-related protein. We previously demonstrated that the Desmoyokin gene is identical to the Ahnak gene, which is expressed ubiquitously and downregulated in neuroblastomas. It was assumed Ahnak/Desmoyokin was associated with epidermal cell adhesion, tumorigenesis, cell proliferation and differentiation, and embryonic development. To determine the precise biological function of Ahnak/Desmoyokin, we generated a null mutation in ES cells and mice. The resultant Ahnak/Desmoyokin-deficient ES cells normally differentiated into embryoid bodies and neural cells. The mutant mice were viable and fertile and showed no gross developmental defects. Electron microscopic examination of skin sections demonstrated that the ultrastructure of epidermal intercellular junctions, including desmosomes, of the mutant mice was indistinguishable from that of wild-type mice. Two-stage chemical skin carcinogenesis experiments showed no difference in frequency or onset of cutaneous tumor formation between wild-type and mutant mice. Moreover, no tumorigenesis was observed in other tissues and organs of mutant mice up to 2 y of age. These results lead us to conclude that Ahnak/Desmoyokin deficiency has only a minimal effect on epidermal cell adhesion, tumorigenesis, cell proliferation and differentiation, and overall mouse development.
机译：Desmoyokin首先从牛口表皮中分离出来，被认为是一种与表皮桥粒相关的蛋白质。我们以前证明了Desmoyokin基因与Ahnak基因相同，后者在神经母细胞瘤中普遍表达并下调。假定Ahnak / Desmoyokin与表皮细胞粘附，肿瘤发生，细胞增殖和分化以及胚胎发育有关。为了确定Ahnak / Desmoyokin的确切生物学功能，我们在ES细胞和小鼠中产生了无效突变。所得的Ahnak /去皮肌激素缺陷型ES细胞通常分化为类胚体和神经细胞。突变小鼠是活的和可育的，并且没有显示出明显的发育缺陷。皮肤切片的电子显微镜检查表明，突变小鼠的表皮细胞间连接（包括桥粒）的超微结构与野生型小鼠没有区别。两阶段化学皮肤癌变实验表明，野生型和突变型小鼠的皮肤肿瘤形成频率或发作没有差异。此外，在2岁以下的突变小鼠的其他组织和器官中均未观察到肿瘤发生。这些结果使我们得出结论，Ahnak / Desmoyokin缺乏症对表皮细胞粘附，肿瘤发生，细胞增殖和分化以及小鼠整体发育的影响很小。

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