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首页> 外文期刊>The Journal of investigative dermatology. >Transforming growth factor-beta receptor type I and type II expression during murine hair follicle development and cycling.
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Transforming growth factor-beta receptor type I and type II expression during murine hair follicle development and cycling.

机译：在鼠毛囊发育和循环过程中转化生长因子-β受体的I型和II型表达。

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Although the TGF-beta family of growth factors probably regulates skin and hair follicle development, its exact role is still quite ill-defined. Here, we characterize the correlative expression pattern of the interdependent high affinity receptor proteins for TGF-beta1 and TGF-beta3, TGF-beta receptor type I (TGF-betaRI) and TGF-beta receptor type II (TGF-betaRII), during hair follicle development and cycling in C57BL/6 mice. During neonatal follicle development, TGF-betaRII immunoreactivity is confined to epithelial cells. Focal epidermal TGF-betaRII expression is seen even before actual hair placode formation. In contrast to the TGF-betaRII immunoreactivity in the outer root sheath, precortical hair matrix and inner root sheath cells were TGF-betaRII negative during hair bulb morphogenesis. TGF-betaRI (Alk-5) immunoreactivity largely overlapped the TGF-betaRII expression pattern, but was more widespread. During hair follicle cycling in adolescent mice, TGF-betaRII immunoreactivity was restricted to follicles, and was strikingly hair cycle dependent (maximal immunoreactivity: anagen VI and early catagen). Again, TGF-betaRI (Alk-5) immunoreactivity co-localized with TGF-betaRII immunoreactivity, but was more extensive. Reverse transcriptase polymerase chain reaction analysis of TGF-betaRII mRNA confirmed peak transcript levels in back skin with most hair follicles in the anagen VI-catagen transformation. mRNA levels of TGF-betaRI (Alk-5) did not vary significantly during the hair cycle, whereas those of TGF-betaRI (threonine-serine kinase 7 L) declined during early anagen, and were maximal during the anagen-catagen transition. This provides a basis for defining the choreography of TGF-beta-related signalling during hair follicle morphogenesis and cycling, introduces intraepidermal TGF-betaRII immunoreactivity as a marker for imminent follicle development, and supports the concept that both TGF-betaRII and TGF-betaRI stimulation is involved in, but not restricted to, the control of catagen induction.
机译：尽管TGF-beta生长因子家族可能调节皮肤和毛囊的发育，但其确切作用仍不清楚。在这里，我们表征毛发期间TGF-beta1和TGF-beta3，TGF-beta受体I型（TGF-betaRI）和TGF-beta受体II型（TGF-betaRII）的相互依赖的高亲和力受体蛋白的相关表达模式C57BL / 6小鼠的卵泡发育和循环。在新生儿卵泡发育过程中，TGF-βRII的免疫反应性仅限于上皮细胞。甚至在实际的头发斑块形成之前就可以看到局灶性表皮TGF-betaRII表达。与外根鞘中的TGF-βRII免疫反应相反，在毛鳞茎形态发生过程中，皮层前毛发基质和内根鞘细胞均为TGF-βRII阴性。 TGF-betaRI（Alk-5）免疫反应性在很大程度上与TGF-betaRII表达模式重叠，但分布更为广泛。在青春期小鼠的毛囊循环过程中，TGF-βRII的免疫反应性仅限于卵泡，并且显着依赖于毛发循环（最大免疫反应性：生长期VI和早期催化作用）。同样，TGF-betaRI（Alk-5）免疫反应性与TGF-betaRII免疫反应性共定位，但范围更广。 TGF-βRIImRNA的逆转录酶聚合酶链反应分析证实，在生长期VI-催化转化中，大多数毛囊的背部皮肤中的转录水平达到峰值。在毛发周期中，TGF-betaRI（Alk-5）的mRNA水平没有显着变化，而TGF-betaRI（苏氨酸-丝氨酸激酶7 L）的mRNA在生长期早期下降，并且在生长期-生长期过渡期间最高。这为定义毛囊形态发生和循环过程中TGF-beta相关信号的编排提供了基础，引入了表皮内TGF-betaRII免疫反应性作为即将到来的卵泡发育的标志，并支持TGF-betaRII和TGF-betaRI刺激的概念参与但不限于控制催化作用。

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