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首页> 外文期刊>The Journal of laboratory and clinical medicine >Assessment of the trypsin-like human prostatic kallikrein, also known as hK2, in the seminal plasma of infertile men: respective contributions of an ELISA procedure and of Western blotting (see comments)
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Assessment of the trypsin-like human prostatic kallikrein, also known as hK2, in the seminal plasma of infertile men: respective contributions of an ELISA procedure and of Western blotting (see comments)

机译：不育男性精浆中胰蛋白酶样人前列腺激肽释放酶（也称为hK2）的评估：ELISA程序和蛋白质印迹的各自贡献（参见评论）

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摘要

Human seminal plasma (SP) is a unique source of kallikreins. Prostate-specific antigen (hK3), which is a chymotrypsin-like human prostatic kallikrein (CHPK), and its cousin protein (hK2), which is recognized as a trypsin-like human prostatic kallikrein (THPK), have been assessed in infertility disorders to test the hypothesis that oligoasthenoteratozoospermia (OAT) is associated with an abnormal prostatic function. Monoclonal antibodies specific for THPK (hK2) were produced by Immunova, Canada, and used to develop a new enzyme-linked immunosorbent assay procedure and to perform Western blot analyses in SP. The immunoradiometric assay from Hybritech Inc., San Diego, Calif., was selected for CHPK (hK3) measurements in SP. Determinations of the THPK and of CHPK contents in SP from four groups of subjects were performed after validation of the assays. The concentration of both kallikreins was similar in three groups of infertile men, and no statistical difference from the control group was recorded. Western blot analysis confirmed the existence of different molecular forms of both kallikreins in SP. Generally, these molecular forms were not affected by infertility disorders except when obstructive azoospermia led to the exclusion of seminal vesicles, which are the sources of protein C inhibitor (PCI). No THPK-PCI complex was observed because THPK, unlike CHPK, is bound mainly to PCI within a few minutes after ejaculation. These data suggest that measurements of kallikreins in the SP of infertile men are much less useful than evaluation of their different molecular forms. Specifically, the absence of THPK-PCI appears to be a reliable feature of obstructive azoospermia, and this test should be routinely practiced in andrology laboratories.
机译：人精浆（SP）是激肽释放酶的独特来源。前列腺特异性抗原（hK3）是一种胰凝乳蛋白酶样的人类前列腺激肽释放酶（CHPK），其表亲蛋白（hK2）是一种胰蛋白酶样的人类前列腺激肽释放酶（THPK），已在不育症中进行了评估检验少脂非小动物精子症（OAT）与前列腺功能异常有关的假说。对THPK（hK2）特异的单克隆抗体由加拿大Immunova生产，用于开发新的酶联免疫吸附测定方法并在SP中进行Western blot分析。选择加利福尼亚州圣地亚哥的Hybritech Inc.的免疫放射分析法进行SP中的CHPK（hK3）测量。验证分析后，对四组受试者的SP中THPK和CHPK含量进行测定。三组不育男性中两种激肽释放酶的浓度相似，与对照组相比无统计学差异。 Western印迹分析证实了SP中两种激肽释放酶的不同分子形式的存在。通常，这些分子形式不受不孕症的影响，除非阻塞性无精子症导致精囊泡被排除，精囊泡是蛋白C抑制剂（PCI）的来源。未观察到THPK-PCI复合物，因为与CHPK不同，THPK主要在射精后几分钟内与PCI结合。这些数据表明，对不育男性SP中激肽释放酶的测定远没有评估其不同分子形式有用。具体而言，不存在THPK-PCI似乎是阻塞性无精子症的可靠特征，因此该检查应在男科实验室常规进行。

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