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首页> 外文期刊>The Journal of Neuroscience: The Official Journal of the Society for Neuroscience >Superoxide production in rat hippocampal neurons: selective imaging with hydroethidine.
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Superoxide production in rat hippocampal neurons: selective imaging with hydroethidine.

机译：大鼠海马神经元中超氧化物的产生：氢乙啶选择性成像。

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摘要

Digital-imaging microfluorimetry of the oxidation of hydroethidine (HEt) to ethidium can be used to monitor superoxide (O2-) production selectively within individual rat hippocampal pyramidal neurons in culture and in brain slices. Under assay conditions, oxidation was not accomplished by hydroxyl radical, singlet O2, H2O2, or nitrogen radicals. Neuronal O2- production varied with metabolic activity and age. O2- generation increased after treatment with AMPA, kainic acid, and NMDA, and the mitochondrial uncoupler carbonylcyanide p-(trifluoromethoxy)phenyl hydrazone, but usually not after depolarization (50 mM K+). O2- concentrations were sensitive to scavengers and nitric oxide. HEt oxidation was higher in Ca(2+)-containing versus Ca(2+)-free saline. However, Ca2+ ionophores did not increase oxidation greatly. H2O2 application produced a secondary increase in O2-. The major source of O2- under basal and stimulated conditions appeared to be the mitochondria. Consistent with this, ethidium staining in dendrites was punctate, colocalized with mitochondria, and blocked by CN-.
机译：氢乙啶（HEt）氧化为乙二胺的数字成像微荧光法可用于选择性监测培养物中和大脑切片中单个大鼠海马锥体神经元内的超氧化物（O2-）产生。在测定条件下，羟基自由基，单线态O2，H2O2或氮自由基无法完成氧化。神经元O2的产生随代谢活性和年龄的变化而变化。用AMPA，海藻酸和NMDA和线粒体解偶联剂羰基氰化物对-（三氟甲氧基）苯基处理后，O2的产生增加，但去极化（50 mM K +）后通常不增加。 O2浓度对清除剂和一氧化氮敏感。与不含Ca（2+）的盐水相比，含Ca（2+）的HEt氧化更高。然而，Ca 2+离子载体并没有大大增加氧化。 H2O2的使用产生了O2-的二次增加。在基础和刺激条件下，O2的主要来源似乎是线粒体。与此相一致，树突中的乙锭染色呈点状，与线粒体共定位，并被CN-阻断。

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