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首页> 外文期刊>The Journal of Neuroscience: The Official Journal of the Society for Neuroscience >Dynamics of target recognition by interstitial axon branching along developing cortical axons.
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Dynamics of target recognition by interstitial axon branching along developing cortical axons.

机译：沿发展中的皮质轴突的间质轴突分支的目标识别动力学。

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Corticospinal axons innervate their midbrain, hindbrain, and spinal targets by extending collateral branches interstitially along their length. To establish that the axon shaft rather than the axonal growth cone is responsible for target recognition in this system, and to characterize the dynamics of interstitial branch formation, we have studied this process in an in vivo-like setting using slice cultures from neonatal mice containing the entire pathway of corticospinal axons. Corticospinal axons labeled with the dye 1,1'-dioctodecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (or Dil) were imaged using time-lapse video microscopy of their pathway overlying the basilar pons, their major hindbrain target. The axon shaft millimeters behind the growth cone exhibits several dynamic behaviors, including the de novo formation of varicosities and filopodia-like extensions, and a behavior that we term "pulsation," which is characterized by a variable thickening and thining of short segments of the axon.An individual axon can have multiple sites of branching activity, with many of the branches being transient. These dynamic behaviors occur along the portion of the axon shaft overlying the basilar pons, but not just caudal to it. Once the collaterals extend into the pontine neuropil, they branch further in the neuropil, while the parent axon becomes quiescent. Thus, the branching activity is spatially restricted to specific portions of the axon, as well as temporally restricted to a relatively brief time window. These findings provide definitive evidence that collateral branches form de novo along corticospinal axons and establish that the process of target recognition in this system is a property of the axon shaft rather than the leading growth cone.
机译：皮质脊髓轴突通过沿其长度间隙性地延伸侧支来支配其中脑，后脑和脊柱靶标。为了确定轴突轴而不是轴突生长锥负责该系统中的目标识别，并表征间质分支形成的动力学，我们使用来自新生小鼠的切片培养物在类似于活体内的环境中研究了该过程皮质脊髓轴突的整个路径。使用延时的视频显微镜对其覆盖基底脑桥（其主要后脑靶标）的路径进行成像，对标记有染料1,1'-dioctodecyl-3,3,3'，3'-四甲基吲哚碳菁高氯酸盐（或Dil）的皮质脊髓轴突进行成像。生长锥后面的轴突轴毫米显示出几种动态行为，包括静脉曲张的从头形成和丝状伪足状延伸，以及我们称之为“脉动”的行为，其特征是短节段的可变增厚和变薄。轴突：单个轴突可以具有多个分支活动位点，其中许多分支是瞬时的。这些动力学行为沿着轴突轴的覆盖基底pons的部分发生，而不仅仅是在其尾部。一旦侧支伸入桥脑神经桩，它们在神经桩中进一步分支，而亲本轴突变得静止。因此，分支活动在空间上限于轴突的特定部分，并且在时间上限于相对短暂的时间窗。这些发现提供了确凿的证据，表明沿皮质脊髓轴突从头开始形成了侧支，并证实了该系统中的目标识别过程是轴突轴的特性，而不是前导生长锥。

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