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首页> 外文期刊>The Journal of Neuroscience: The Official Journal of the Society for Neuroscience >Reciprocal interactions between neurons and glia are required for Drosophila peripheral nervous system development.
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Reciprocal interactions between neurons and glia are required for Drosophila peripheral nervous system development.

机译：果蝇外周神经系统发育需要神经元和神经胶质之间的相互作用。

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A major developmental role of peripheral glia is to mediate sensory axon guidance; however, it is not known whether sensory neurons influence peripheral glial development. To determine whether glia and neurons reciprocally interact during embryonic development, we ablated each cell type by overexpressing the apoptosis gene, grim, and observed the effects on peripheral nervous system (PNS) development. When neurons are ablated, glial defects occur as a secondary effect, and vice versa. Therefore glia and neurons are codependent during embryogenesis. To further explore glial-neuronal interactions, we genetically disrupted glial migration or differentiation and observed the secondary effects on sensory neuron development. Glial migration and ensheathment of PNS axons was blocked by overexpression of activated Rho GTPase, a regulator of actin dynamics. Here, sensory axons extended to the CNS without exhibiting gross pathfinding errors. In contrast, disrupting differentiation by expression of dominant-negative Ras GTPase in glia resulted in major sensory axon pathfinding errors, similar to those seen in glial ablations. Glial overexpression of transgenic components of the epidermal growth factor receptor (EGFR) signaling pathway yielded similar sensory neuron defects and also downregulated the expression of the glial marker Neuroglian. Mutant analysis also suggested that the EGFR ligands Spitz and Vein play roles in peripheral glial development. The observations support a model in which glia express genes necessary for sensory neuron development, and these genes are potentially under the control of the EGFR/Ras signaling pathway.
机译：周围神经胶质细胞的主要发展作用是介导感觉轴突的引导。然而，尚不清楚感觉神经元是否影响周围神经胶质的发育。为了确定神经胶质和神经元在胚胎发育过程中是否相互相互作用，我们通过过度表达凋亡基因，严峻来消融每种细胞类型，并观察其对周围神经系统（PNS）发育的影响。当神经元被消融时，神经胶质缺损会作为次要作用发生，反之亦然。因此，胶质细胞和神经元在胚胎发生过程中是相互依赖的。为了进一步探索神经胶质与神经元的相互作用，我们从遗传上破坏了神经胶质的迁移或分化，并观察了对感觉神经元发育的次级影响。 PNS轴突的胶质迁移和鞘被活化的Rho GTPase（肌动蛋白动力学的调节剂）的过表达所阻断。在这里，感觉轴突延伸到中枢神经系统，而没有表现出明显的寻路误差。相反，通过在胶质细胞中表达显性阴性的Ras GTPase破坏分化，会导致严重的感觉轴突寻路错误，与神经胶质消融相似。表皮生长因子受体（EGFR）信号转导途径的转基因成分的神经胶质过度表达产生了类似的感觉神经元缺陷，并且还下调了神经胶质标记Neuroglian的表达。突变分析还表明，EGFR配体Spitz和Vein在周围神经胶质发育中起作用。观察结果支持其中胶质细胞表达感觉神经元发育所必需的基因的模型，并且这些基因可能处于EGFR / Ras信号通路的控制之下。

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