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首页> 外文期刊>The Journal of Neuroscience: The Official Journal of the Society for Neuroscience >Activity-dependent differential transmitter release in mouse adrenal chromaffin cells.
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Activity-dependent differential transmitter release in mouse adrenal chromaffin cells.

机译：小鼠肾上腺嗜铬细胞中活性依赖性差异递质的释放。

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摘要

Chromaffin cells of the adrenal medulla are a primary neuroendocrine output of the sympathetic nervous system. When stimulated, they secrete a host of transmitter molecules, including catecholamines and neuropeptides, through the fusion of dense core secretory granules with the cell surface. At basal firing rates, set by the sympathetic tone, chromaffin cells selectively release catecholamines at a modest rate. Stress-mediated sympathetic activation leads to elevated catecholamine secretion and also evokes neuropeptide release. Catecholamines and neuropeptides are copackaged in the same granules; thus, it is unclear how this activity-dependent differential transmitter release is achieved. In this report, we use electrophysiological, electrochemical, fluorescence, and immunocytochemical approaches to quantify transmitter release under physiological electrical stimulation at the single cell level. We provide data to show that chromaffin cells selectively release catecholamine under basal firing conditions but release both neuropeptides and catecholamines under conditions that match acute stress. We further show that this differential transmitter release is achieved through a regulated activity-dependent dilation of the granule fusion pore. Thus, chromaffin cells may regulate release of different transmitters through a simple size-exclusion mechanism.
机译：肾上腺髓质的嗜铬细胞是交感神经系统的主要神经内分泌输出。受到刺激时，它们通过致密的核心分泌颗粒与细胞表面融合，分泌出大量的递质分子，包括儿茶酚胺和神经肽。在由交感神经决定的基础放电速率下，嗜铬细胞以适度的速率选择性地释放儿茶酚胺。应激介导的交感神经激活导致儿茶酚胺分泌增加，并引起神经肽释放。儿茶酚胺和神经肽共同包装在同一颗粒中;因此，尚不清楚如何实现这种取决于活动的差分变送器释放。在本报告中，我们使用电生理，电化学，荧光和免疫细胞化学方法来量化生理电刺激下单细胞水平下的递质释放。我们提供的数据表明，嗜铬细胞在基础激发条件下选择性释放儿茶酚胺，但在与急性应激相匹配的条件下释放神经肽和儿茶酚胺。我们进一步表明，这种差异性的递质释放是通过调节活性依赖的颗粒融合孔的扩张来实现的。因此，嗜铬细胞可通过简单的大小排阻机制调节不同递质的释放。

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