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首页> 外文期刊>The Journal of Neuroscience: The Official Journal of the Society for Neuroscience >Apoptotic death of beta cells after optic nerve transection in adult cats.
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Apoptotic death of beta cells after optic nerve transection in adult cats.

机译：成年猫视神经横断后β细胞的凋亡死亡。

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We have revealed previously that the survival rate of beta cells of cat retinal ganglion cells (RGCs) rapidly decreased to 29% on day 7 after optic nerve transection, whereas that of alpha cells slowly decreased to 64% on day 14 (Watanabe et al., 2001). The reason that beta cells die more rapidly than alpha cells was not clear. In the present study, we tested the possibility that the rapid death of beta cells is attributable to apoptosis, as shown for some axotomized RGCs in rats. The following results were obtained. First, the proportion of pyknotic cells in Nissl-stained cat retinas started to increase sharply starting on day 4 and reached a peak on day 6 after optic nerve transection. The time course of occurrence of pyknotic cells corresponded well with that of the rapid death of axotomized beta cells. Secondly, the proportion of pyknotic cells was the highest in the area centralis (AC), in which beta cells are densely distributed. The preferential death of axotomized RGCs in the AC was also confirmed by terminal deoxynucleotidyl transferase-mediated biotinylated UTP nick end labeling staining in cross sections. Thirdly, after the intravitreal injection of caspase 3 inhibitor (z-DEVD-cmk) the survival of axotomized beta cells on day 7 was significantly enhanced, whereas no such survival-promoting effect was obtained in axotomized alpha cells. Taken together, these results suggest that the rapid death of axotomized beta cells is attributable mainly to apoptosis, which is mediated by caspase 3.
机译：先前我们已经发现，视神经横断后第7天，猫视网膜神经节细胞（RGCs）β细胞的存活率迅速降低至29％，而在第14天，α细胞的存活率缓慢降低至64％（Watanabe等。 ，2001）。 β细胞比α细胞死亡更快的原因尚不清楚。在本研究中，我们测试了β细胞快速死亡归因于细胞凋亡的可能性，如大鼠中一些经轴突切除的RGC所示。获得了以下结果。首先，尼氏染色的猫视网膜中的泌尿生殖细胞比例从第4天开始急剧增加，并在视神经横断后第6天达到峰值。私密性细胞的发生时间与轴突切除的β细胞迅速死亡的时间相吻合。其次，在β区密集分布的中央区域（AC）中，泌尿生殖细胞的比例最高。还通过末端脱氧核苷酸转移酶介导的生物素化UTP缺口末端标记染色证实了AC中轴切RGC的优先死亡。第三，玻璃体内注射半胱天冬酶3抑制剂（z-DEVD-cmk）后，第7天，被轴突切除的β细胞的存活率显着提高，而在被轴突切除的α细胞中未获得这种存活促进作用。两者合计，这些结果表明，轴突切除的β细胞的快速死亡主要归因于凋亡，这是由胱天蛋白酶3介导的。

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