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首页> 外文期刊>The Journal of Neuroscience: The Official Journal of the Society for Neuroscience >Lighting up the senses: FM1-43 loading of sensory cells through nonselective ion channels.
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Lighting up the senses: FM1-43 loading of sensory cells through nonselective ion channels.

机译：照亮感觉：FM1-43通过非选择性离子通道装载感觉细胞。

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摘要

We describe a novel mechanism for vital fluorescent dye entry into sensory cells and neurons: permeation through ion channels. In addition to the slow conventional uptake of styryl dyes by endocytosis, small styryl dyes such as FM1-43 rapidly and specifically label hair cells in the inner ear by entering through open mechanotransduction channels. This labeling can be blocked by pharmacological or mechanical closing of the channels. This phenomenon is not limited to hair cell transduction channels, because human embryonic kidney 293T cells expressing the vanilloid receptor (TRPV1) or a purinergic receptor (P2X2) rapidly take up FM1-43 when those receptor channels are opened and not when they are pharmacologically blocked. This channel permeation mechanism can also be used to label many sensory cell types in vivo. A single subcutaneous injection of FM1-43 (3 mg/kg body weight) in mice brightly labels hair cells, Merkel cells, muscle spindles, taste buds, enteric neurons, and primary sensory neurons within the cranial and dorsal root ganglia, persisting for several weeks. The pattern of labeling is specific; nonsensory cells and neurons remain unlabeled. The labeling of the sensory neurons requires dye entry through the sensory terminal, consistent with permeation through the sensory channels. This suggests that organic cationic dyes are able to pass through a number of different sensory channels. The bright and specific labeling with styryl dyes provides a novel way to study sensory cells and neurons in vivo and in vitro, and it offers new opportunities for visually assaying sensory channel function.
机译：我们描述了一种重要的荧光染料进入感觉细胞和神经元的新机制：通过离子通道的渗透。除了通过内吞作用缓慢吸收苯乙烯基染料的常规方法外，小的苯乙烯基染料（例如FM1-43）还可以通过开放的机械转导通道快速进入内耳并特别标记内耳的毛细胞。该标记可以通过药理或机械封闭通道来阻止。这种现象不仅限于毛细胞转导通道，因为表达vanilloid受体（TRPV1）或嘌呤能受体（P2X2）的人胚胎肾脏293T细胞在打开这些通道时会迅速吸收FM1-43，而在药理上被阻断时则不会。该通道渗透机制还可以用于标记体内许多感觉细胞类型。在小鼠中单次皮下注射FM1-43（体重3 mg / kg）可以清楚地标记出颅神经和背根神经节内的毛细胞，默克尔细胞，肌梭，味蕾，肠神经元和初级感觉神经元。周。标签的模式是特定的;非感觉细胞和神经元仍未标记。感觉神经元的标记需要染料通过感觉末端进入，这与通过感觉通道的渗透一致。这表明有机阳离子染料能够通过许多不同的感觉通道。苯乙烯基染料的明亮而特异性的标记为在体内和体外研究感觉细胞和神经元提供了一种新颖的方法，并且为视觉检测感觉通道功能提供了新的机会。

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