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首页> 外文期刊>The Journal of Neuroscience: The Official Journal of the Society for Neuroscience >Olig transcription factors are expressed in oligodendrocyte and neuronal cells in human fetal CNS.
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Olig transcription factors are expressed in oligodendrocyte and neuronal cells in human fetal CNS.

机译：寡转录因子在人胎儿CNS的少突胶质细胞和神经元细胞中表达。

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摘要

The transcription factors Olig1 and Olig2 are closely associated with the development of oligodendrocyte (OL) lineage in the vertebrate nervous system, but little is known about their role in the human developing CNS. To test the hypothesis that they contribute to initial OL specification in humans, we studied the expression of Olig1 and Olig2 in human fetuses at 5-24 gestational weeks (GW). Both transcription factors were present in well outlined regions of the ventral neuroepithelium at 5 GW, several weeks before oligodendrogenesis. Spatial differences in the expression of Olig1 and Olig2 along the neuronal axis suggest that they specify different subpopulations of progenitor cells. Olig1 was distributed rostrally, from the basal forebrain to the hindbrain, whereas Olig2 was also found in the ventral spinal cord. Furthermore, at 5 GW, Olig1 was coexpressed with vimentin, and Olig2 was coexpressed with a neuronal marker, microtubule-associated protein 2. With the progression of development at 15 GW, both proteins were present throughout the spinal cord and the ventricular-subventricular zone of the ganglionic eminences, whereas at midgestation (20 GW), they were also expressed in the telencephalic proliferative zones and the emerging white matter. Double-labeling studies revealed that early OL progenitor cells and radial glia expressed Olig1, whereas Olig2 was localized predominantly in mature OLs and a subset of neural progenitor cells and mature neurons. Thus, Olig1 and Olig2 transcription factors in the human CNS are important not only for differentiation of the OL lineage, but they may also have a role in neural cell specification.
机译：转录因子Olig1和Olig2与脊椎动物神经系统中少突胶质细胞（OL）谱系的发育密切相关，但对其在人类发展中枢神经系统中的作用了解甚少。为了检验它们有助于人类最初的OL规范的假设，我们研究了在5-24个孕周（GW）时人类胎儿中Olig1和Olig2的表达。这两种转录因子均在少突胶质形成前几周出现在5 GW的腹侧神经上皮轮廓清晰的区域中。沿着神经元轴的Olig1和Olig2表达的空间差异表明，它们指定了祖细胞的不同亚群。 Olig1分布在从前脑基底到后脑的后方，而Olig2也见于腹侧脊髓。此外，在5 GW时，Olig1与波形蛋白共表达，而Olig2与神经元标志物，微管相关蛋白2共表达。随着15 GW的发展，两种蛋白都存在于整个脊髓和心室-心室区的神经节隆起，而在妊娠中期（20 GW），它们也在端脑增生区和新出现的白质中表达。双重标记研究表明，早期OL祖细胞和放射状神经胶质细胞表达Olig1，而Olig2主要位于成熟的OLs和神经祖细胞和成熟的神经元中。因此，人CNS中的Olig1和Olig2转录因子不仅对于OL谱系的分化很重要，而且在神经细胞规范中也可能起作用。

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