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首页> 外文期刊>The Journal of Neuroscience: The Official Journal of the Society for Neuroscience >A novel antigen sensitive to calcium chelation that is associated with the tip links and kinocilial links of sensory hair bundles.
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A novel antigen sensitive to calcium chelation that is associated with the tip links and kinocilial links of sensory hair bundles.

机译：一种新型的对钙螯合敏感的抗原，与感觉性发束的尖端连接和纤毛连接相关。

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摘要

Tip links are extracellular, cell-surface-associated filaments of unknown molecular composition that are thought to gate the mechanotransducer channel of the sensory hair cell. They disappear from the hair bundle in response to calcium chelation and lanthanum treatment and resist degradation by the protease subtilisin. A monoclonal antibody derived from a hybridoma screen identified a novel antigen associated with tip links, the tip-link antigen. The tip-link antigen is also associated with kinocilial links, subtilisin-resistant filaments that are sensitive to calcium chelation and connect the kinocilium to the tallest stereocilia of the hair bundle. Furthermore, the tip-link antigen is expressed in the retina, where it is associated with the ciliary calyx, a ring of microvilli that surrounds the outer segment of the photoreceptor. The tip-link antigen rapidly disappears from the surface of the hair bundle in response to calcium chelation. It is also subtilisin resistant, relative to the ankle-link antigen, an antigen associated with another type of hair bundle link. The tip-link antigen is lanthanum sensitive and, like tip links, reappears on the surface of the hair bundle after calcium chelation. The monoclonal antibody to the tip-link antigen immunoprecipitates two concanavalin A-reactive polypeptides with apparent molecular masses of 200 and 250 kDa from detergent extracts of the retina. These results provide the first identification of a cell surface antigen associated with tip links, indicate that tip links share properties in common with kinocilial links, and reveal a second epitope that, along with the ankle-link antigen, is common to both sensory hair bundles and the ciliary calyx of photoreceptors.
机译：尖端连接是未知分子组成的细胞外，与细胞表面相关的细丝，被认为是控制感觉毛细胞的机械换能器通道的通道。它们响应钙螯合和镧处理而从发束中消失，并抵抗蛋白酶枯草杆菌蛋白酶降解。源自杂交瘤筛选的单克隆抗体鉴定了与尖端连接相关的新型抗原，即尖端连接抗原。尖端连接抗原还与肌动蛋白链相关联，是耐枯草杆菌蛋白酶的细丝，对钙螯合敏感，并将运动菌毛与发束的最高立体纤毛相连。此外，尖端连接抗原在视网膜中表达，在视网膜中与睫状花萼相关联，睫状花萼是微绒毛状的环，围绕着感光体的外部。响应钙螯合，尖端链接抗原迅速从发束表面消失。相对于脚踝链接抗原，它也是一种枯草杆菌蛋白酶抗性，该抗原与另一种类型的发束链接相关。尖端连接抗原对镧敏感，并且与尖端连接一样，在钙螯合后重新出现在发束的表面。尖端连接抗原的单克隆抗体从视网膜的洗涤剂提取物中免疫沉淀出两种伴刀豆球蛋白A反应性多肽，其表观分子量分别为200和250 kDa。这些结果首次鉴定了与末端连接有关的细胞表面抗原，表明末端连接与链状连接具有共同的特性，并揭示了第二个表位，与脚踝连接抗原一起对于两个感官发束都是相同的。和感光细胞的睫状花萼。

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