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首页> 外文期刊>The Journal of Neuroscience: The Official Journal of the Society for Neuroscience >Transmembrane sema4E guides branchiomotor axons to their targets in zebrafish.
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Transmembrane sema4E guides branchiomotor axons to their targets in zebrafish.

机译：跨膜sema4E将分支运动轴突引导至斑马鱼的靶标。

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摘要

Class 4 semaphorins are a large class of transmembrane proteins that contain a sema domain and that are expressed in the CNS, but their in vivo neural function is unknown. In zebrafish, the epithelial cells that line the pharyngeal arches express Sema4E. Extension of branchiomotor axons along the mesenchymal cells bounded by these epithelial cells suggests that Sema4E may act as a repulsive guidance molecule to restrict the branchiomotor axons to the mesenchymal cells. To test this hypothesis, Sema4E was misexpressed in hsp70 promoter-regulated transgenic zebrafish in which sema4E was heat-inducible, and Sema4E was knocked down by injection of antisense morpholino oligonucleotides that acted specifically against Sema4E. Ubiquitous induction of Sema4E retarded outgrowth by the facial and gill branchiomotor axons significantly. Furthermore, outgrowth by gill motor axons was specifically inhibited when Sema4E-expressing transgenic cells were transplanted to their pathway in nontransgenic host embryos. Morpholino knockdown of Sema4E caused facial motor axons to defasciculate and follow aberrant pathways. These results show that Sema4E is repulsive for facial and gill motor axons and functions as a barrier for these axons within the pharyngeal arches.
机译：4类信号量蛋白是一大类跨膜蛋白，它们包含一个信号量域并在CNS中表达，但其体内神经功能尚不清楚。在斑马鱼中，排列在咽弓上的上皮细胞表达Sema4E。分支运动轴突沿由这些上皮细胞结合的间充质细胞的延伸表明，Sema4E可能充当排斥性指导分子，以将分支运动轴突限制于间充质细胞。为了验证这一假设，在HSP70启动子调控的转基因斑马鱼中Sema4E的表达不正确，在该斑马鱼中sema4E是可热诱导的，并且通过注射特异针对Sema4E的反义吗啉代寡核苷酸敲低了Sema4E。无处不在的Sema4E诱导显着延缓了面部和腮部分支运动轴突的生长。此外，当表达Sema4E的转基因细胞被移植到非转基因宿主胚胎的途径中时，g运动轴突的生长被特异性抑制。 Sema4E的Morpholino抑制导致面部运动轴突去角质并遵循异常途径。这些结果表明，Sema4E对面部和腮部运动轴突具有排斥力，并且可作为咽弓内这些轴突的屏障。

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