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首页> 外文期刊>The Journal of Neuroscience: The Official Journal of the Society for Neuroscience >Cortical plaque-like structures identify ribosome-containing domains in the Mauthner cell axon.
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Cortical plaque-like structures identify ribosome-containing domains in the Mauthner cell axon.

机译：皮质斑块样结构识别Mauthner细胞轴突中的含核糖体域。

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摘要

The hypothesis that ribosomes are present, but may have a restricted distribution, in the Mauthner (M) axon was evaluated in isolated M-cell axoplasm after (1) staining with YOYO-1 and (2) inspection by electron spectroscopic imaging (ESI) of ribosomal RNA (rRNA) phosphorus (P). Discrete periaxoplasmic plaques, identified by their ribonuclease-sensitive fluorescence, were located circumferentially at the surface boundary of isolated axoplasm and distributed longitudinally at random intervals. Conditions that destabilized plaques, and surface blotting of plaques onto a coverslip, revealed that fluorescent puncta were probably a significant source of plaque fluorescence. Fluorescent puncta were also distributed in a delimited volume of axoplasm, subjacent to the plaque. The notably higher density of F-actin in the latter region suggested that the actin cytoskeleton may govern the spatial distribution of puncta in subcortical axoplasm. Some fluorescent plaques were superficial to the cortical F-actin layer, whereas others formed inclusions within the F-actin layer; however, plaques did not appear to contain F-actin. Periaxoplasmic plaques were also identified in ordinary myelinated axons. ESI, in which rRNA emits bright signals in the phosphorus (P) spectral line against a low-contrast background, showed that isolated axoplasm contained characteristic 25 nm P signals, which were associated or in direct contact with a pleiomorphic structural matrix, located at the surface boundary. Polyribosomal P signals were also distributed in peripheral axoplasm below the matrix. The concept of a distinct polyribosome-populated domain, distributed intermittently in the cortical zone of the axon is described. This domain is spatially defined by a plaque-like periaxoplasmic structural matrix, and a confluent volume of subcortical axoplasm integrated through an actin cytoskeleton.
机译：在（1）用YOYO-1染色和（2）通过电子光谱成像（ESI）检查之后，在分离的M细胞轴突中评估了Mauthner（M）轴突中存在核糖体但可能具有受限分布的假说。核糖体RNA（rRNA）磷（P）。由核糖核酸酶敏感的荧光鉴定出的离散轴周质斑在周向上位于分离的轴质的表面边界处，并以随机间隔纵向分布。使斑块不稳定并使斑块表面印迹到盖玻片上的条件表明，荧光点可能是斑块荧光的重要来源。荧光点也分布在一定数量的腋窝中，紧邻斑块。在后者区域，F-肌动蛋白的密度显着较高，表明肌动蛋白的细胞骨架可能决定皮层腋窝腋窝点的空间分布。一些荧光斑块位于皮质F-肌动蛋白层的表面，而其他荧光斑则在F-肌动蛋白层内形成夹杂物。但是，噬菌斑似乎不包含F-肌动蛋白。在普通的髓鞘轴突中也鉴定出了eria浆菌斑。 ESI，其中rRNA在低对比度背景下的磷（P）谱线中发出明亮的信号，表明分离的质体包含特征性的25 nm P信号，这些信号与位于其表面的多形结构基质相关或直接接触。表面边界。多核糖体P信号也分布在基质下方的周围轴质中。描述了一个独特的多核糖体密集域的概念，该域间歇性分布在轴突的皮质区。该区域在空间上由斑块状轴突质结构基质和通过肌动蛋白细胞骨架整合的汇合体积的皮层下轴突质定义。

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