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首页> 外文期刊>The Journal of Neuroscience: The Official Journal of the Society for Neuroscience >Mechanisms underlying developmental changes in the firing patterns of ON and OFF retinal ganglion cells during refinement of their central projections.
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Mechanisms underlying developmental changes in the firing patterns of ON and OFF retinal ganglion cells during refinement of their central projections.

机译：在其中央投影的细化过程中，ON和OFF视网膜神经节细胞的放电模式发展变化的潜在机制。

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Patterned neuronal activity is implicated in the refinement of connectivity during development. Calcium-imaging studies of the immature ferret visual system demonstrated previously that functionally separate ON and OFF retinal ganglion cells (RGCs) develop distinct temporal patterns of spontaneous activity as their axonal projections undergo refinement. OFF RGCs become spontaneously more active compared with ON cells, resulting in a decrease in synchronous activity between these cell types. This change in ON and OFF activity patterns is suitable for driving the activity-dependent refinement of their axonal projections. Here, we used whole-cell and perforated-patch recording techniques to elucidate the mechanisms that underlie the developmental alteration in the ON and OFF RGC activity patterns. First, we show that before the refinement period, ON and OFF RGCs have similar spike patterns; however, during the period of segregation, OFF RGCs demonstrate significantly higher spike rates relative to ON cells. With increasing age, OFF cells require less depolarization to reach their action potential threshold and fire more spikes in response to current injection compared with ON cells. In addition, spontaneous postsynaptic currents and potentials are greater in magnitude in OFF cells than ON cells. In contrast, before axonal refinement, there are no differences in the intrinsic excitability or synaptic drive onto ON and OFF cells. Together, our results show that developmental changes in ON and OFF RGC excitability and in the strength of their synaptic drives act together to reshape the spike patterns of these cells in a manner appropriate for the refinement of their connectivity.
机译：模式化的神经元活动与发育过程中的连通性细化有关。未成熟的雪貂视觉系统的钙成像研究先前表明，功能分离的ON和OFF视网膜神经节细胞（RGC）在其轴突投影经过精细化处理时会形成不同的自发活动时间模式。与ON细胞相比，OFF RGC自发地更具活性，导致这些细胞类型之间的同步活性降低。开和关活动模式的这种变化适合于驱动依赖于其轴突投影的活动性细化。在这里，我们使用了全细胞和穿孔膜片记录技术来阐明在ON和OFF RGC活性模式中发生发育变化的机制。首先，我们表明在细化周期之前，ON和OFF RGC具有相似的尖峰模式。然而，在分离期间，相对于ON细胞，OFF RGC表现出明显更高的刺突率。随着年龄的增长，与ON细胞相比，OFF细胞需要更少的去极化才能达到其动作电位阈值，并响应电流注入而发射更多的尖峰。另外，OFF细胞中的自发突触后电流和电位在幅度上大于ON细胞。相反，在轴突细化之前，内在兴奋性或突触驱动到ON和OFF细胞上没有差异。总之，我们的结果表明，ON和OFF RGC兴奋性的发展变化及其突触驱动强度共同作用，以适合于改善其连通性的方式重塑了这些细胞的尖峰模式。

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