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首页> 外文期刊>Chromatographia >Development of an LC Method for Simultaneous Analysis of Cinnamic Acid
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Development of an LC Method for Simultaneous Analysis of Cinnamic Acid

机译：液相色谱法同时分析肉桂酸的方法开发

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A specific and accurate high-performance liquid chromatographic method for analysis of cinnamic acid (CA) and paeonol (PN) in rat plasma has been developed and validated. Plasma samples were pretreated by protein precipitation with methanol, and the supernatant was injected for reversed-phase separation on a C-18 column with acetonitrile-0.1% phosphoric acid 24:76 (v/v) as mobile phase at a flow-rate of 1.0 mL min(-1). Phenylbutyric acid was used as the internal standard. Good linear relationships were obtained between response and concentration in the range 0.130-52.0 mu g mL(-1) (r = 0.9980) and 0.1785-71.4 mu g mL(-1) (r = 0.9950) for CA and PN, respectively. Intra-day and inter-day assay precision (RSD, n = 6) at three concentrations was not above 15.1% for either CA and PN, and accuracy was from 94.3 to 104.7% and from 103.3 to 113.1% for CA and PN, respectively. Mean recovery of CA and PN from plasma samples was 87.5 and 86.8%, respectively, and recovery of the internal standard at a concentration of 1.00 mg mL(-1) was 88.5%. Results from a stability study suggested CA and PN were stable under the experimental conditions used. Finally, the validated method was successfully applied to a pharmacokinetic study of CA and PN in rats after intragastric administration of Guizhi-Fuling capsule. The results obtained would be very useful for evaluation of the clinical efficacy of GFC.
机译：已经开发并验证了一种用于分析大鼠血浆中肉桂酸（CA）和丹皮酚（PN）的特定且准确的高效液相色谱方法。血浆样品用甲醇进行蛋白沉淀预处理，然后将上清液注入C-18色谱柱中，以乙腈-0.1％磷酸24:76（v / v）作为流动相，反相分离。 1.0 mL分钟（-1）。苯丁酸用作内标。对于CA和PN，在浓度和响应范围分别为0.130-52.0μg mL（-1）（r = 0.9980）和0.1785-71.4μg mL（-1）（r = 0.9950）之间获得了良好的线性关系。在三种浓度下，CA和PN的日内和日间测定精度（RSD，n = 6）均不高于15.1％，CA和PN的准确度分别为94.3％至104.7％和103.3％至113.1％ 。血浆样品中CA和PN的平均回收率分别为87.5和86.8％，内标浓度为1.00 mg mL（-1）的回收率为88.5％。稳定性研究的结果表明，CA和PN在使用的实验条件下是稳定的。最终，该验证方法成功地应用于桂枝弗灵胶囊灌胃后大鼠体内CA和PN的药代动力学研究。获得的结果对于评估GFC的临床疗效非常有用。

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