Studies of the rate constant of L-DOPA oxidation and decarboxylation by HPLC

Zhou Y.Z.; Alany R.G.; Chuang V.; Wen J.

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Studies of the rate constant of L-DOPA oxidation and decarboxylation by HPLC

机译：HPLC研究L-DOPA氧化脱羧速率常数

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The objective of current investigation was to study the degradation behavior of L-DOPA under different conditions by high performance liquid chromatography (HPLC), and to develop and validate a stability-indicating HPLC method. The developed RP-HPLC method was validated with respect to linearity, accuracy, precision and specificity. Oxidation was found to occur in alkaline and to some extent in thermal conditions, while the drug was stable when incubated at acidic conditions and under photolytic stress. The oxidation of L-DOPA was observed to follow first-order kinetics. The degradation rate constants and half-life were calculated. The cytotoxicity and enzymatic degradation of L-DOPA was examined using the human intestinal epithelial Caco-2 cells. The drug was rapidly decarboxylated by aromatic amino acid decarboxylase to dopamine. The conversion of L-DOPA to dopamine was dose- and time-dependent.

机译：当前研究的目的是通过高效液相色谱（HPLC）研究L-DOPA在不同条件下的降解行为，并开发和验证稳定性指示HPLC方法。所开发的RP-HPLC方法在线性，准确性，精密度和特异性方面得到了验证。发现在碱性条件下以及在一定程度上在热条件下都会发生氧化，而在酸性条件下和在光解应力下孵育时该药物是稳定的。观察到L-DOPA的氧化遵循一级动力学。计算降解速率常数和半衰期。使用人肠上皮Caco-2细胞检查L-DOPA的细胞毒性和酶促降解。该药物被芳香族氨基酸脱羧酶迅速脱羧成多巴胺。 L-DOPA向多巴胺的转化是剂量和时间依赖性的。

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《Chromatographia》 |2012年第12期|共10页
作者
Zhou Y.Z.; Alany R.G.; Chuang V.; Wen J.;
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正文语种 eng
中图分类分析化学;
关键词
Column liquid chromatography; Decarboxylation; L-DOPA; Oxidation; Rate constant;

机译：柱液相色谱;脱羧;L-DOPA;氧化;速率常数;

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Studies of the rate constant of L-DOPA oxidation and decarboxylation by HPLC

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