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首页> 外文期刊>Biochimica et biophysica acta. Molecular basis of disease: BBA >Ethanol-induced augmentation of annexin IV in cultured cells and the enhancement of cytotoxicity by overexpression of annexin IV by ethanol
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Ethanol-induced augmentation of annexin IV in cultured cells and the enhancement of cytotoxicity by overexpression of annexin IV by ethanol

机译：乙醇诱导培养细胞中膜联蛋白IV的增加和乙醇过表达膜联蛋白IV引起的细胞毒性增强

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摘要

The annexins are a family of highly homologous Ca~(2+) and phospholipid binding proteins. The expressive amounts of several annexins have been shown to alter in certain pathological states such as brain ischemia and Alzheimer's disease. It has been demonstrated that ethanol induces cytotoxicity, which results in brain damage. In this study, we examined the relationship between ethanol-induced cytotoxicity and the intrinsic amount of annexins using cell lines (rat glioma C6 cells and human adenocarcinoma A549 cells). A decrease in the mitochondrial enzyme (dehydrogenase) activity, which is widely used to measure cytotoxicity, was observed with a high concentration of ethanol (200 mM or more) after a 24-h exposure in both C6 and A549 cells. Western blot analysis revealed that the amount of annexin IV was augmented in both cells by ethanol, whereas levels of annexins I and V were unchanged. The amount of annexin IV was augmented with increasing concentration of ethanol. The overexpression of annexin IV in C6 cells by transfection with annexin IV-DNA enhanced ethanol-induced cell lesion and was accompanied by NFKB activation. Thus, it might be indicated that the amount of annexin IV is selectively augmented and this augmentation facilitates the development of cell lesion by ethanol.
机译：Annexins是一类高度同源的Ca〜（2+）和磷脂结合蛋白。某些膜联蛋白的表达量已显示在某些病理状态下发生变化，例如脑缺血和阿尔茨海默氏病。已经证明乙醇诱导细胞毒性，这导致脑损伤。在这项研究中，我们使用细胞系（大鼠神经胶质瘤C6细胞和人腺癌A549细胞）检查了乙醇诱导的细胞毒性与膜联蛋白固有量之间的关系。在C6和A549细胞中暴露24小时后，高浓度乙醇（200 mM或更高）观察到广泛用于测量细胞毒性的线粒体酶（脱氢酶）活性降低。蛋白质印迹分析表明，乙醇可增加两种细胞中膜联蛋白IV的量，而膜联蛋白I和V的水平却没有变化。随着乙醇浓度的增加，膜联蛋白IV的量增加。通过膜联蛋白IV-DNA转染在C6细胞中膜联蛋白IV的过表达增强了乙醇诱导的细胞损伤，并伴有NFKB激活。因此，可能表明膜联蛋白IV的量被选择性地增加，并且这种增加促进了乙醇对细胞损伤的发展。

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